MMR/CD206/Mannose Receptor Antibody (685641)

Western blot shows lysates of human immature dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25342) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMR/CD206 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human monocyte-derived immature dendritic cells were stained with Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25342, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 685641
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MMR/CD206/Mannose Receptor Antibody (685641) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
  Leu19-Lys1383 (Thr399Ala) & (Leu407Phe)
  Accession # P22897
• Specificity: Detects human MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse MMR is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MRC1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMR/CD206/Mannose Receptor Antibody (685641)

• CD206
• CLEC13D
• CLEC13Dmacrophage mannose receptor 1
• C-type lectin domain family 13 member D
• mannose receptor, C type 1
• MMR
• MMRCD206 antigen
• MRC1

Background

The human Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 190 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The human MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1371 aa extracellular region, a 21 aa transmembrane segment and a 46 aa cytoplasmic domain (4). Its extracellular region is composed of an N‑terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3, 4). Human to mouse, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N‑acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (5). Several of the CRDs participate in the Ca2⁺-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (6). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin‑coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor is recycled to the cell surface (3, 7). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
4. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
5. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
6. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
7. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMR/CD206/Mannose Receptor Antibody (MAB25342)(2)

Publications using MAB25342

• S He, C Wu, J Xiao, D Li, Z Sun, M Li Endothelial extracellular vesicles modulate the macrophage phenotype: Potential implications in atherosclerosis Scand. J. Immunol., 2018-04-01;0(0):. 2018-04-01 [PMID: 29465752] (Flow Cytometry, Human)
• Nandakumar , Subhadra, Kannanganat , Sunil, Dobos , Karen M, Lucas , Megan, Spencer , John S, Fang , Sunan, McDonald , Melissa, Pohl , Jan, Birkness , Kristin, Chamcha , Venkates, Ramirez , Melissa, Plikaytis , Bonnie B, Posey , James E, Amara , Rama Rao, Sable , Suraj B O-mannosylation of the Mycobacterium tuberculosis adhesin Apa is crucial for T cell antigenicity during infection but is expendable for protection. PLoS Pathog, 2013-10-10;9(10):e1003705. 2013-10-10 [PMID: 24130497] (Functional Assay, Human)

Bioinformatics

• Gene Symbol: MRC1
• Uniprot:
  • P22897()