Human MMP-9 ELISA Kit (Colorimetric)

ELISA: MMP-9 ELISA Kit [Biotin] [NBP1-82422] - Standard curve

Product Discontinued

Human MMP-9 ELISA Kit (Colorimetric) Summary

• Description: This ELISA kit has been transitioned to a new version, available here: DMP900
• Specificity: The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human MMP-9 positive sample. There was no cross reactivity or interference detected.
• Standard Curve Range: 0.23 - 15.0 ng/mL
• Sensitivity: 0.05 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10.2%
• Intra-Assay: CV% < 7.3%
• Spike Recovery: Recoveries were determined in 2 independent experiments with 4 replicates each. The overall mean recovery was 103.1%.
• Sample Volume: 10 uL (prediluted 1 : 25)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MMP9

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human MMP-9 ELISA Kit (Colorimetric)

• 92 kDa gelatinase
• 92 kDa type IV collagenase
• CLG4B
• EC 3.4.24
• EC 3.4.24.35
• Gelatinase B
• GELB
• macrophage gelatinase
• MANDP2
• matrix metallopeptidase 9
• matrix metalloproteinase 9
• matrix metalloproteinase-9
• MMP9
• MMP-9
• type V collagenase

Background

MMP-9 is a Zn+2 dependent endopeptidase, synthesized and secreted in monomeric form as zymogen. The structure is almost similar to MMP2, another member of matrixmetalloproteinase family. Like other proteolytic enzymes, MMP-9 is first synthesized as inactive proenzyme or zymogens. Activation of proMMP-9 is mediated by plasminogen activator/plasmin (PA/plasmin) system. The regulation of MMP-9 activity is also controlled through TIMP-3. MMP-9 plays an important role in tumor invasion, angiogenesis and metastasis. Increased levels of MMP-9 have been demonstrated in colorectal cancer, in acute leukemia, breast cancer, human melanoma and bladder cancer. MMP-9 potentiates pulmonary metastasis formation and glioma invasion. Apart from its function in tumor formation and spread, MMP-9 with its destructive effects is in close association with lung diseases and asthma. Leukocyte recruitment to the central nervous system MMP-9 means an involvement in the pathogenesis of multiple sclerosis. MMP-9 has a crucial role in reproductive endocrinology and shows a changed expression pattern after myocardial infarction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for MMP-9 ELISA Kit (NBP1-82422). (Showing 1 - 2 of 2 FAQ).

1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
   • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MMP9