MMP-9 Antibody Pair [HRP]

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Sandwich ELISA: MMP-9 Antibody Pair [HRP] [NBP2-79671] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications S-ELISA
Clonality
Monoclonal
Conjugate
HRP
Standard Curve Range
125-8000 pg/ml
Sensitivity
31.25 pg/ml

Order Details

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Catalog# & Conjugate Size Price

MMP-9 Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human MMP-9.
Specificity
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human MMP-9.
Standard Curve Range
125-8000 pg/ml
Sensitivity
31.25 pg/ml
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Clonality
Monoclonal
Gene
MMP9

Applications/Dilutions

Application Notes
1. Capture Antibody: Dilute to a working concentration of 1 ug/ml in PBS before coating.
2. Detection Antibody: Dilute to working concentration of 0.1 ug/ml in Dilution Buffer before use.
3. Standard: Reconstitute with 1 ml Dilution Buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 8000 pg/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Standard: Each vial contains 80 ng of recombinant Human MMP-9.
  2. Detection Antibody: 0.2 mg/ml of rabbit anti-Human MMP-9 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  3. Capture Antibody: 1 mg/ml of mouse anti-Human MMP-9 monoclonal antibody (in PBS, pH 7.4).

Alternate Names for MMP-9 Antibody Pair [HRP]

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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FAQs for MMP-9 Antibody Pair (NBP2-79671). (Showing 1 - 1 of 1 FAQ).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&applications=ELISA&common_name=MMP-9It looks like 2 have been tested for capture (please note the tested species for each of these).https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Capture%20(Matched%20Antibody%20Pair)1 has been tested for detection.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Detection%20(Matched%20Antibody%20Pair)

Other Available Formats

HRP NBP2-79671
HRP NBP2-79671

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Bioinformatics

Gene Symbol MMP9