MMP-9 Antibody (5G3)

Images

 
Western Blot: MMP-9 Antibody (5G3) [NBP2-22181] - Western blot analysis using MMP9 mAb against human MMP9 (AA: 238-465) recombinant protein. (Expected MW is 50.6 kDa)
Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody (5G3) [NBP2-22181] - Immunofluorescence analysis of NIH/3T3 cells using MMP9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry-Paraffin: MMP-9 Antibody (5G3) [NBP2-22181] - Immunohistochemical analysis of paraffin-embedded brain tissues using MMP9 mouse mAb with DAB staining.
Flow Cytometry: MMP-9 Antibody (5G3) [NBP2-22181] - Flow cytometric analysis of Hela cells using MMP9 mouse mAb (blue) and negative control (red).
Western Blot: MMP-9 Antibody (5G3) [NBP2-22181] - Western blot analysis using MMP9 mAb against HEK293 (1), MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate.
ELISA: MMP-9 Antibody (5G3) [NBP2-22181] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
5G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MMP-9 Antibody (5G3) Summary

Immunogen
Purified recombinant fragment of human MMP9 expressed in E. Coli.
Localization
Cytoplasm
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MMP9
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for MMP-9 Antibody (5G3)

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on MMP-9.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol MMP9
Entrez