MMP-9 Antibody (5G3)

Western Blot: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis using MMP9 mAb against human MMP9 (AA: 238-465) recombinant protein. (Expected MW is 50.6 kDa)

Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of NIH/3T3 cells using MMP9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of tissues using MMP9 mouse mAb with DAB staining.

Flow Cytometry: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of Hela cells using MMP9 mouse mAb (blue) and negative control (red).

Western Blot: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis using MMP9 mAb against HEK293 (1), MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate.

ELISA: MMP-9 Antibody (5G3) [NBP2-22181] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, KD
• Clone: 5G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MMP-9 Antibody (5G3) Summary

• Immunogen: Purified recombinant fragment of human MMP-9 expressed in E. Coli.
• Localization: Cytoplasm
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP9
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:2000
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for MMP-9 Antibody (5G3)

• 92 kDa gelatinase
• 92 kDa type IV collagenase
• CLG4B
• EC 3.4.24
• EC 3.4.24.35
• Gelatinase B
• GELB
• macrophage gelatinase
• MANDP2
• matrix metallopeptidase 9
• matrix metalloproteinase 9
• matrix metalloproteinase-9
• MMP9
• MMP-9
• type V collagenase

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-9 Antibody (NBP2-22181)(1)

Publication using NBP2-22181

• Shang S, Ji X, Zhang L et al. Macrophage ABHD5 suppresses NF-kappa B-dependent matrix metalloproteinase expression and cancer metastasis Cancer Res. 2019-08-22 [PMID: 31439546] (KD, ELISA, Mouse)

FAQs for MMP-9 Antibody (NBP2-22181). (Showing 1 - 1 of 1 FAQ).

1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
   • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

Bioinformatics

• Gene Symbol: MMP9