Rat MMP-17 ELISA Kit (Colorimetric)

ELISA: Rat MMP-17 ELISA Kit (Colorimetric) [NBP3-06890] - Samples were spiked with high concentrations of Rat MMP-17 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat MMP-17 ELISA Kit (Colorimetric) [NBP3-06890] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Rat MMP-17 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat MMP-17 in samples. No significant cross-reactivity or interference between Rat MMP-17 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 5.28
• Intra-Assay: 5.3
• Spike Recovery: 86-108 %
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MMP17

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat MMP-17 ELISA Kit (Colorimetric)

• matrix metallopeptidase 17 (membrane-inserted)
• matrix metalloproteinase 17 (membrane-inserted)
• matrix metalloproteinase-17
• membrane-type matrix metalloproteinase 4
• membrane-type-4 matrix metalloproteinase
• MMP17
• MMP-17
• MT4MMP
• MT4-MMP
• MT-MMP 4
• MTMMP4

Background

Membrane type (MT) matrix metalloproteinases (MMPs) are recently recognized members of the family of Zn(2+)- and Ca(2+)-dependent MMPs. MMP-17 is, therefore, a competent Zn(2+)-dependent MMP with unique specificity among synthetic substrates and the capability to both degrade gelatin and activate progelatinase A (1). (MMP-17) is expressed mainly in the brain, leukocytes, the colon, the ovary, and the testis. The expression of MMP-17 in leukocytes together with its putative membrane localization suggest that this enzyme could be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators such as tumor necrosis factor-alpha (2). glycosylphospha-tidylinositol (GPI) anchoring of MT4-MMP on the cell surface indicates a unique biological function and character for this proteinase (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for MMP-17 ELISA Kit (NBP3-06890). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MMP17