MMP-17 Antibody (JU33-03)

Immunocytochemistry/Immunofluorescence: MMP-17 Antibody (JU33-03) [NBP2-75574] - Staining MMP17 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MMP-17 Antibody (JU33-03) [NBP2-75574] - Analysis of paraffin-embedded mouse brain tissue using anti-MMP17 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: MMP-17 Antibody (JU33-03) [NBP2-75574] - Staining MMP17 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

MMP-17 Antibody (JU33-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human MMP-17 aa 175-225 / 603. (SwissProt: Q9ULZ9 Human; SwissProt: Q9R0S3 Mouse)
• Localization: Secreted. Plasma membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MMP17
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MMP-17 Antibody (JU33-03)

• matrix metallopeptidase 17 (membrane-inserted)
• matrix metalloproteinase 17 (membrane-inserted)
• matrix metalloproteinase-17
• membrane-type matrix metalloproteinase 4
• membrane-type-4 matrix metalloproteinase
• MMP17
• MMP-17
• MT4MMP
• MT4-MMP
• MT-MMP 4
• MTMMP4

Background

Membrane type (MT) matrix metalloproteinases (MMPs) are recently recognized members of the family of Zn(2+)- and Ca(2+)-dependent MMPs. MMP-17 is, therefore, a competent Zn(2+)-dependent MMP with unique specificity among synthetic substrates and the capability to both degrade gelatin and activate progelatinase A (1). (MMP-17) is expressed mainly in the brain, leukocytes, the colon, the ovary, and the testis. The expression of MMP-17 in leukocytes together with its putative membrane localization suggest that this enzyme could be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators such as tumor necrosis factor-alpha (2). glycosylphospha-tidylinositol (GPI) anchoring of MT4-MMP on the cell surface indicates a unique biological function and character for this proteinase (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MMP17