Human MMP-15/MT2-MMP ELISA Kit (Colorimetric)

ELISA: Human MMP-15/MT2-MMP ELISA Kit (Colorimetric) [NBP3-06940] - Samples were spiked with high concentrations of Human MMP-15/MT2-MMP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human MMP-15/MT2-MMP ELISA Kit (Colorimetric) [NBP3-06940] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Human MMP-15/MT2-MMP ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human MMP-15/MT2-MMP in samples. No significant cross-reactivity or interference between Human MMP-15/MT2-MMP and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 4.47
• Intra-Assay: 4.95
• Spike Recovery: 92-110 %
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MMP15

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human MMP-15/MT2-MMP ELISA Kit (Colorimetric)

• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.80
• matrix metallopeptidase 15 (membrane-inserted)
• matrix metalloproteinase 15 (membrane-inserted)
• Membrane-type matrix metalloproteinase 2
• Membrane-type-2 matrix metalloproteinase
• MMP15
• MMP-15
• MT2-MMP
• MT2-MMPMT2MMP
• MTMMP2MT-MMP 2
• SMCP-2matrix metalloproteinase-15

Background

MMPs are a group of enzymes involved in matrix degradation. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT MMPs exist as integral membrane proteins. MT1 MMp, MT2 MMP and MT3 MMP also known as MMP 14, MMP 15 and MMP 16 respectively. They contain a C-terminal transmembrane domain anchoring them to the cell surface. MT2 MMP plays a minor role in activation of pro-MMP 2 to its active form in breast carcinomas. Coexpression of MT1 MMP and MT2 MMP in advanced stages of breast carcinomas is indicative of possible involvement of MT2 MMP in its metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for MMP-15/MT2-MMP ELISA Kit (NBP3-06940). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MMP15