Human MMP-15/MT2-MMP ELISA Kit (Colorimetric)

ELISA: Human MMP-15/MT2-MMP ELISA Kit (Colorimetric) [NBP3-06940] - Samples were spiked with high concentrations of Human MMP-15/MT2-MMP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human MMP-15/MT2-MMP ELISA Kit (Colorimetric) [NBP3-06940] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Human MMP-15/MT2-MMP ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human MMP-15/MT2-MMP in samples. No significant cross-reactivity or interference between Human MMP-15/MT2-MMP and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 4.47
• Intra-Assay: 4.95
• Spike Recovery: 92-110 %
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MMP15

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human MMP-15/MT2-MMP ELISA Kit (Colorimetric)

• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.80
• matrix metallopeptidase 15 (membrane-inserted)
• matrix metalloproteinase 15 (membrane-inserted)
• Membrane-type matrix metalloproteinase 2
• Membrane-type-2 matrix metalloproteinase
• MMP15
• MMP-15
• MT2-MMP
• MT2-MMPMT2MMP
• MTMMP2MT-MMP 2
• SMCP-2matrix metalloproteinase-15

Background

MMPs are a group of enzymes involved in matrix degradation. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT MMPs exist as integral membrane proteins. MT1 MMp, MT2 MMP and MT3 MMP also known as MMP 14, MMP 15 and MMP 16 respectively. They contain a C-terminal transmembrane domain anchoring them to the cell surface. MT2 MMP plays a minor role in activation of pro-MMP 2 to its active form in breast carcinomas. Coexpression of MT1 MMP and MT2 MMP in advanced stages of breast carcinomas is indicative of possible involvement of MT2 MMP in its metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MMP15