MMP-14/MT1-MMP Antibody [Unconjugated]

MDA-MB-231 human breast cancer cell line was stained with Goat Anti-Human MMP-14/MT1-MMP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF918, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MMP-14/MT1-MMP Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP‑14/MT1‑MMP
• Specificity: Detects human MMP‑14/MT1‑MMP in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MMP14
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-14/MT1-MMP Antibody [Unconjugated]

• EC 3.4.24
• EC 3.4.24.80
• matrix metallopeptidase 14 (membrane-inserted)
• matrix metalloproteinase 14 (membrane-inserted)
• matrix metalloproteinase-14
• membrane type 1 metalloprotease
• Membrane-type matrix metalloproteinase 1
• Membrane-type-1 matrix metalloproteinase
• MMP14
• MMP-14
• MMP-X1
• MT1MMP
• MT1-MMP
• MT1-MMPMTMMP1
• MT-MMP 1
• MT-MMP1

Background

As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alpha _V (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (2). Recombinant Human MMP-14 consists of the pro and catalytic domains.

1. Seike, M. (2003) Cancer Lett. 194:1.
   
2. Sato, H. et al. (1994) Nature 370:61.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-14/MT1-MMP Antibody (AF918)(2)

Publications using AF918

• Gkantidis , Nikolaos, Blumer , Susan, Katsaros , Christos, Graf , Daniel, Chiquet , Matthias Site-Specific Expression of Gelatinolytic Activity during Morphogenesis of the Secondary Palate in the Mouse Embryo. PLoS ONE, 2012-10-16;7(10):e47762. 2012-10-16 [PMID: 23091646] (IHC-Fr, Mouse)
• Fisher KE, Pop A, Koh W Tumor cell invasion of collagen matrices requires coordinate lipid agonist-induced G-protein and membrane-type matrix metalloproteinase-1-dependent signaling. Mol. Cancer, 2006-12-08;5(0):69. 2006-12-08 [PMID: 17156449] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MMP14