MMP-14/MT1-MMP Antibody (114-6G6) - Azide Free

Immunohistochemistry: MMP-14/MT1-MMP Antibody (114-6G6) [NBP2-29699] - Mouse anti-MT1-MMP Staining of breast carcinoma.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 114-6G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free
• Concentration: 2 mg/ml

MMP-14/MT1-MMP Antibody (114-6G6) - Azide Free Summary

• Description: Relevant Entrez Gene Numbers: NM_004995.2
• Immunogen: This antibody is specific to MMP-14
• Specificity: The antibody specifically reacts with human MT1-MMP. This is a purified mouse monoclonal antibody to an oligopeptide of REVPYAYIREGHEK (residue 160-173) on human membrane-type 1 matrix metalloproteinase (human MT1-MMP) and does not react with mouse or rat. However, this sequence is identical in mouse and rat MT1-MMP. For unknown reasons this antibody does not react on westerns to CC1043.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP14
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 8-20 ug/ml
• Application Notes: ImmunoBlotting: 66+60kDa (Ueno et al., 1997) Immunohistochemistry on paraffin-embedded tissue sections, use at 20 ug/mL concentration (see Ueno et al., 1997; Nakamura et al., 1999). Requires periodate-lysine-paraformaldehyde fixative (Namura et al., 1999) for 18-24 hrs. at 4C. EIA

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Sodium Phosphate (pH 7.0) and 2% BSA (protease free)
• Preservative: No Preservative
• Concentration: 2 mg/ml
• Purity: Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_004995.2

Alternate Names for MMP-14/MT1-MMP Antibody (114-6G6) - Azide Free

• EC 3.4.24
• EC 3.4.24.80
• matrix metallopeptidase 14 (membrane-inserted)
• matrix metalloproteinase 14 (membrane-inserted)
• matrix metalloproteinase-14
• membrane type 1 metalloprotease
• Membrane-type matrix metalloproteinase 1
• Membrane-type-1 matrix metalloproteinase
• MMP14
• MMP-14
• MMP-X1
• MT1MMP
• MT1-MMP
• MT1-MMPMTMMP1
• MT-MMP 1
• MT-MMP1

Background

The matrix metalloproteinase (MMP) family (approximately 25 members in mammals) has been implicated in extracellular matrix remodeling associated with embryonic development, cancer formation and progression, and various other physiological and pathological events. MMP-14 is a membrane-bound matrix metalloproteinase (MT-MMP) capable of mediating pericellular proteolysis of extracellular matrix components. MMP-14 is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix (1). Gelatinase A (type-IV collagenase; M(r) 72,000) is produced by tumour stroma cells and is believed to be crucial for their invasion and metastasis, acting by degrading extracellular matrix macro-molecules such as type IV collagen. MMP-14 may thus trigger invasion by tumor cells by activating pro-gelatinase A on the tumor cell surface (2). Observations identify MT1-MMP as a tumor-derived growth factor that regulates proliferation by controlling cell geometry within the confines of the 3D extracellular matrix (ECM) (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-14/MT1-MMP Antibody (NBP2-29699)(1)

Publication using NBP2-29699

• Popa GV, Costache A, Badea O et al. Histopathological and immunohistochemical study of periodontal changes in chronic smokers Romanian Journal of Morphology and Embryology 2021-10-10 [PMID: 34609423] (Immunohistochemistry)

Bioinformatics

• Gene Symbol: MMP14
• Uniprot:
  • P50281()