MMP-13 Antibody

Western Blot: MMP-13 Antibody [NBP2-17310] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: MMP-13 Antibody [NBP2-17310] - NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: MMP13 protein stained by MMP13 antibody [N3C1], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: MMP-13 Antibody [NBP2-17310] - Analysis of paraffin-embedded Lung AdCA, using antibody at 1:250 dilution.

• Reactivity: Hu, Rt, Rb
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MMP-13 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MMP13. The exact sequence is proprietary.
• Localization: Secreted; extracellular space; extracellular matrix
• Predicted Species: Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MMP13
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28265573).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
• Preservative: 0.01% Thimerosal
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MMP-13 Antibody

• CLG 3
• CLG3EC 3.4.24
• collagenase 3
• EC 3.4.24.-
• EC 3.4.24.22
• EC 3.4.24.24
• EC 3.4.24.35
• EC 3.4.24.65
• EC 3.4.24.7
• MANDP1
• matrix metallopeptidase 13 (collagenase 3)
• matrix metalloproteinase 13 (collagenase 3)
• Matrix metalloproteinase-13
• MMP13
• MMP-13

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-13 Antibody (NBP2-17310)(1)

Publication using NBP2-17310

• Huang W, Ao P, Li J et al. Autophagy protects advanced glycation end product-induced apoptosis and. BioMed Research International. Jan 16 2017 [PMID: 28265573] (WB, Rat)

Bioinformatics

• Gene Symbol: MMP13
• Entrez:
  • 4322(Human)
• Uniprot:
  • P45452()
  • NM_002427(Human)