![Western Blot: MMP-13 Antibody [NBP2-17310] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/MMP-13-Antibody-Western-Blot-NBP2-17310-img0005.jpg "Western Blot: MMP-13 Antibody [NBP2-17310] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE gel, diluted at 1:1000.")
| Reactivity | Hu, Rt, RbSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MMP13. The exact sequence is proprietary. |
| Localization | Secreted; extracellular space; extracellular matrix |
| Predicted Species | Rabbit (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MMP13 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 54 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Immunogen affinity purified |
| Publication using NBP2-17310 | Applications | Species |
|---|---|---|
| Huang W, Ao P, Li J et al. Autophagy protects advanced glycation end product-induced apoptosis and. BioMed Research International. Jan 16 2017 [PMID: 28265573] (WB, Rat) | WB | Rat |
Secondary Antibodies |
Isotype Controls |
Diseases for MMP-13 Antibody (NBP2-17310)Discover more about diseases related to MMP-13 Antibody (NBP2-17310).
| Pathways for MMP-13 Antibody (NBP2-17310)View related products by pathway.
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PTMs for MMP-13 Antibody (NBP2-17310)Learn more about PTMs related to MMP-13 Antibody (NBP2-17310).
| Research Areas for MMP-13 Antibody (NBP2-17310)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: MMP-13 Antibody [NBP2-17310] - NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: MMP13 protein stained by MMP13 antibody [N3C1], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize2/MMP-13-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17310-img0004.jpg "Immunocytochemistry/Immunofluorescence: MMP-13 Antibody [NBP2-17310] - NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: MMP13 protein stained by MMP13 antibody [N3C1], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: MMP-13 Antibody [NBP2-17310] - Analysis of paraffin-embedded Lung AdCA, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize2/MMP-13-Antibody-Immunohistochemistry-Paraffin-NBP2-17310-img0006.jpg "Immunohistochemistry-Paraffin: MMP-13 Antibody [NBP2-17310] - Analysis of paraffin-embedded Lung AdCA, using antibody at 1:250 dilution.")
![Western Blot: MMP-2 Antibody - (Pro and Active) [NB200-193] - WB analysis of recombinant pro and active forms of Human MMP-2 protein (left lane) and crude homogenate of the injured Rat peripheral nerve (right lane) using MMP-2 antibody at 0.5 ug/ml concentration. The antibody detected both pro as well as cleaved/active forms of MMP-2.](http://images.novusbio.com/fullsize2/MMP-2-Antibody---(Pro-and-Active)-Western-Blot-NB200-193-img0012.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody - (Pro and Active) [NB200-193] - IHC analysis of a formalin fixed paraffin embedded tissue section of human hepatocellular carcinoma using at 1:400 dilution with HRP-conjugated secondary antibody and DAB based detection. Hematoxylin counterstaining was performed to visualize nuclei and the antibody was found to generate a diffused but specific staining of MMP2 protein in the cytoplasm and the inter-cellular spaces of the hepatic cancer cells.](http://images.novusbio.com/fullsize2/MMP-2-Antibody---(Pro-and-Active)-Immunohistochemistry-Paraffin-NB200-193-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: MMP-2 Antibody - (Pro and Active) [NB200-193] - analysis of MMP-2 in HeLa cells using anti-MMP-2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/MMP-2-Antibody---(Pro-and-Active)-Immunocytochemistry-Immunofluorescence-NB200-193-img0011.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Western blot analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.](http://images.novusbio.com/fullsize2/c-jun-Antibody-(5B1)-Western-Blot-NBP2-37576-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Immunofluorescence analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize2/c-jun-Antibody-(5B1)-Immunocytochemistry-Immunofluorescence-NBP2-37576-img0004.jpg)
![Immunohistochemistry: c-jun Antibody (5B1) [NBP2-37576] - Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/c-jun-Antibody-(5B1)-Immunohistochemistry-NBP2-37576-img0007.jpg)
![Western Blot: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in L929 whole cell extract using NB100-74350.](http://images.novusbio.com/fullsize2/Aggrecan Neoepitope Antibody-Western Blot-NB100-74350-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Aggrecan Neoepitope Antibody [NB100-74350] - Detection of ACAN in OA cartilage using NB100-74350.](http://images.novusbio.com/fullsize2/Aggrecan Neoepitope Antibody-Immunocytochemistry-Immunofluorescence-NB100-74350-img0007.jpg)
![Immunohistochemistry: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in mouse cartilage with hematoxylin counterstain.](http://images.novusbio.com/fullsize2/Aggrecan-Neoepitope-Antibody-Immunohistochemistry-NB100-74350-img0008.jpg)
![Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Top panel: Analysis of c-Fos expression in HeLa cells using NBP2-50037. Lane 1: HeLa cells were serum-starved for 36 hours. Lane 2: Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. NBP2-50037 recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos. Serum starvation attenuates c-Fos expression, while 20% FBS strongly stimulates c-Fos expression. Bottom panel: Blot was stripped and probed with monoclonal antibody against GAPDH (NB300-221) used as loading control.](http://images.novusbio.com/fullsize2/c-Fos-Antibody-(2H2)-Western-Blot-NBP2-50037-img0003.jpg)
![Immunocytochemistry: c-Fos Antibody (2H2) [NBP2-50037] - pAb 1:1000 (green), DAPI counterstain (blue) on 30 micron cryosection of mouse spinal cord. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/c-Fos Antibody (2H2)-Immunocytochemistry-NBP2-50037-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation (bottom), or PBS treatment (top). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with chicken pAb against Vimentin (NB300-223, red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 uM; fixed by transcardial perfusion with 4% PFA) labeled with NBP2-50037 using a standard HRP-DAB staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with NBP2-50037 (red) and rabbit polyclonal anti-NeuN (NBP1-92716, green) using IF confocal. Neurons positive for c-Fos and RBFOX3/NeuN appear to be yellow. Inset shows an enlarged image of NBP2-50037 staining. Nuclei are labeled with Dapi (blue).](http://images.novusbio.com/fullsize2/c-Fos-Antibody-(2H2)-Immunocytochemistry-Immunofluorescence-NBP2-50037-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0005.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.")
![SDS-Page: Recombinant Human MMP-13 Protein [NBP1-99622]](http://images.novusbio.com/images2/Recombinant Human MMP-13 Protein-SDS-Page-NBP1-99622-img0001.jpg "SDS-Page: Recombinant Human MMP-13 Protein [NBP1-99622]")