MMP-13 Antibody

Western Blot: MMP-13 Antibody [NBP1-45723] - Analysis of MMP-13 expression with Jurkat cell lysate.

Immunocytochemistry/Immunofluorescence: MMP-13 Antibody [NBP1-45723] - MMP-13 antibody was tested at 1:50 in MDA-MB-231 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.

Western Blot: MMP-13 Antibody [NBP1-45723] - Analysis of MMP-13 in human full-length recombinant protein using anti-MMP-13 antibody. Image from verified customer review.

Simple Western: MMP-13 Antibody [NBP1-45723] - Simple Western lane view shows a specific band for MMP-13 in 0.5 mg/mL of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Bv, Eq, Rb
• Applications: WB, Simple Western, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MMP-13 Antibody Summary

• Immunogen: Synthetic peptide surrounding amino acid 454 of rat MMP-13.
• Specificity: The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MMP13
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Simple Western
  • Western Blot
• Application Notes: Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 25703139).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 5 mM EDTA, 0.5% BSA, 30% Glycerol
• Preservative: 0.03% Proclin 300
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for MMP-13 Antibody

• CLG 3
• CLG3EC 3.4.24
• collagenase 3
• EC 3.4.24.-
• EC 3.4.24.22
• EC 3.4.24.24
• EC 3.4.24.35
• EC 3.4.24.65
• EC 3.4.24.7
• MANDP1
• matrix metallopeptidase 13 (collagenase 3)
• matrix metalloproteinase 13 (collagenase 3)
• Matrix metalloproteinase-13
• MMP13
• MMP-13

Background

Collagenase-3 (MMP13), a member of the matrix metalloproteinase (MMP) family of neutral endopeptidases, is expressed in the skeleton during embryonic development and is highly overexpressed in human carcinomas and in chondrocytes and synovial cells in rheumatoid arthritis and osteoarthritis. Findings demonstrate a unique role of Mmp13 in skeletal development (1). In arthritis, the destruction of fibrillar (type II) collagen is one of the hallmarks, with MMP-1 and MMP-13 being identified as key players in arthritic cartilage. MMP-13, furthermore, has been found in highly metastatic tumors. In contrast to many human MMPs, the MMP-13 distribution pattern is restrictive in normal tissues and selective in pathological conditions. MMP-13 demonstrates versatility in its substrate utilization. In addition to being highly active on type II collagen, MMP-13 cleaves other substrates, mostly macromolecules of the extracellular matrix, but also molecules such as connective tissue (CTGF) and fibrinogen. MMP-13 is controlled at multiple levels: i.e., the expression/synthesis, activation, and inhibition of the active enzyme (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MMP-13 Antibody (NBP1-45723)(8)

Publications using NBP1-45723

• Hyung-Mun Yun, Hyun Sook Kwon, Joon Yeop Lee, Kyung-Ran Park, Antonio González-Sarrías Vitexicarpin Induces Apoptosis and Inhibits Metastatic Properties via the AKT-PRAS40 Pathway in Human Osteosarcoma International Journal of Molecular Sciences 2024-03-22 [PMID: 38612399]
• Ortiz-Sánchez BJ, Juárez-Avelar I, Andrade-Meza A et al. Periodontitis exacerbation during pregnancy in mice: Role of macrophage migration inhibitory factor as a key inductor Journal of periodontal research 2023-11-21 [PMID: 37990413] (IHC, Mouse)
• Xing W, Kesavan C, Pourteymoor S, Mohan S. Global and Conditional Disruption of the Igf-I Gene in Osteoblasts and/or Chondrocytes Unveils Epiphyseal and Metaphyseal Bone-Specific Effects of IGF-I in Bone Biology (Basel) 2023-09-12 [PMID: 37759627] (Western Blot, Immunohistochemistry-Paraffin, Mouse)
• Yun HM, Kim B, Kim SH et al. Xanol Promotes Apoptosis and Autophagy and Inhibits Necroptosis and Metastasis via the Inhibition of AKT Signaling in Human Oral Squamous Cell Carcinoma Cells 2023-07-03 [PMID: 37443802] (Western Blot, Immunohistochemistry-Paraffin, Mouse)
• Shen, L;Sun, Z;Nie, P;Yuan, R;Cai, Z;Wu, C;Hu, L;Jin, S;Zhou, H;Zhang, X;He, B; Sulindac-derived RXR-alpha modulator attenuates atherosclerotic plaque progression and destabilization in ApoE-/- mice Br. J. Pharmacol. 2019-04-03 [PMID: 30943581] (ICC/IF, Mouse)
• Liu W, Xia P, Feng J et al. MicroRNA-132 upregulation promotes matrix degradation in intervertebral disc degeneration Exp. Cell Res. 2017-08-06 [PMID: 28793234] (ICC/IF, Human)
• Ma X, Liu Y, Wang Q et al. Tamoxifen induces the development of hernia in mice by activating MMP-2 and MMP-13 expression Biochim. Biophys. Acta. 2015-02-19 [PMID: 25703139] (IHC-P, WB, Mouse)
• Park K, Kwon Y, Jeong Y et al. Thelephoric acid, p-terphenyl, induces bone-forming activities in pre-osteoblasts Journal of Functional Foods 2022-04-01 (WB, Mouse)

Review for MMP-13 Antibody (NBP1-45723) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/03/2015

Summary

• Application: Western Blot
• Sample Tested: Recombinant protein
• Species: Human

Bioinformatics

• Gene Symbol: MMP13
• Entrez:
  • 281914(Bovine)
  • 4322(Human)
  • 17386(Mouse)
  • 171052(Rat)
• Uniprot:
  • P23097(Rat)