MMP-11 Antibody (8N9D3)

Western Blot: MMP-11 Antibody (8N9D3) [NBP3-15342] - Western blot analysis of extracts of various cell lines, using MMP-11 Rabbit mAb (NBP3-15342) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 8N9D3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MMP-11 Antibody (8N9D3) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 389-488 of human MMP-11 (P24347). AALVWGPEKNKIYFFRGRDYWRFHPSTRRVDSPVPRRATDWRGVPSEIDAAFQDADGYAYFLRGRLYWKFDPVKVKALEGFPRLVGPDFFGCAEPANTFL
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MMP11
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MMP-11 Antibody (8N9D3)

• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.24
• matrix metallopeptidase 11 (stromelysin 3)
• matrix metalloproteinase 11 (stromelysin 3)
• Matrix metalloproteinase-11
• MMP11
• MMP-11
• SL-3
• ST3
• STMY3stromelysin 3
• stromelysin III
• stromelysin-3

Background

The matrix metalloproteinases are a tightly regulated family of enzymes that degrade extracellular matrix and basement membrane components. Recent evidence suggests that these proteases and their specific inhibitors play important roles in normal developmental processes and in pathological conditions (1). MMP-11 (stromelysin-3), a new member of the matrix metalloproteinase family, is expressed in tissues undergoing the active remodeling associated with embryonic development, wound healing and tumor invasion. But like all other members of the matrix metalloproteinase gene family, stromelysin-3 is synthesized as an inactive precursor that must be processed to its mature form in order to express enzymic activity (2). MMP-11, like an inflammatory mediator, may exert a chemotactic influence on macrophages which aggregate in the mesangium; MMP-11 is not likely to have a parallel mitogenic or antifibrotic effect in diseased glomeruli (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MMP11