MMP-10 Antibody (110304)

MMP‑10 was detected in immersion fixed paraffin-embedded sections of human colon cancer using Human MMP‑10 Monoclonal Antibody (Catalog # MAB910) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 110304
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MMP-10 Antibody (110304) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP-10
  Tyr18-Cys476
  Accession # P09238
• Specificity: Detects human MMP-10 in Western blots. Does not cross-react with recombinant human MMP-1, -2, -3, -7, -8, -9, -12, or -13.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP10
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-10 Antibody (110304)

• EC 3.4.24
• EC 3.4.24.22
• matrix metallopeptidase 10 (stromelysin 2)
• matrix metalloprotease 10
• matrix metalloproteinase 10 (stromelysin 2)
• Matrix metalloproteinase-10
• MMP10
• MMP-10
• SL-2
• STMY2stromelysin-2
• Stromelysin 2
• transin 2
• transin-2

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium, and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site; a short linker region, and a carboxyl terminal hemopexin-like domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-10 Antibody (MAB910)(9)

Publications using MAB910

• Li, L;Jiao, L;Feng, D;Yuan, Y;Yang, X;Li, J;Jiang, D;Chen, H;Meng, Q;Chen, R;Fang, B;Zou, X;Luo, Z;Ye, X;Hong, Y;Liu, C;Li, C; Human apical-out nasal organoids reveal an essential role of matrix metalloproteinases in airway epithelial differentiation Nature communications 2024-01-02 [PMID: 38168066] (IHC, Human)
• J Kudelski, G M?ynarczyk, M Gudowska-S, B Mroczko, B Darewicz, M Bruczko-Go, L Romanowicz Higher Content but Not Activity of Stromelysin-2 (MMP-10) in Comparison to Stromelysin-1 (MMP-3) in Human Renal Carcinoma International Journal of Environmental Research and Public Health, 2022-10-02;19(19):. 2022-10-02 [PMID: 36231910] (Western Blot, Human)
• J Kudelski, G M?ynarczyk, M Gudowska-S, B Mroczko, B Darewicz, M Bruczko-Go, K Sobolewski, L Romanowicz Enhanced Expression but Decreased Specific Activity of Matrix Metalloproteinase 10 (MMP-10) in Comparison with Matrix Metalloproteinase 3 (MMP-3) in Human Urinary Bladder Carcinoma Journal of Clinical Medicine, 2021-08-19;10(16):. 2021-08-19 [PMID: 34441979] (Western Blot, Human)
• F Luo, AP Tran, L Xin, C Sanapala, BT Lang, J Silver, Y Yang Modulation of proteoglycan receptor PTP? enhances MMP-2 activity to promote recovery from multiple sclerosis Nat Commun, 2018-10-08;9(1):4126. 10/8/2018 [PMID: 30297691]
• Puram Sidharth V, Tirosh Itay, Parikh Anuraag S et al. Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer. Cell 12/14/2018 [PMID: 29198524]
• Jameson T. Crowley, Klemen Strle, Elise E. Drouin, Annalisa Pianta, Sheila L. Arvikar, Qi Wang et al. Matrix metalloproteinase-10 is a target of T and B cell responses that correlate with synovial pathology in patients with antibiotic-refractory Lyme arthritis Journal of Autoimmunity 2016-05-01 [PMID: 26922382]
• Du X, Lin B, Wang Q, Li H, Ingalla E, Tien J, Rooney I, Ashkenazi A, Penuel E, Qing J MMP-1 and Pro-MMP-10 as potential urinary pharmacodynamic biomarkers of FGFR3-targeted therapy in patients with bladder cancer. Clin Cancer Res, 2014-10-17;20(24):6324-35. 2014-10-17 [PMID: 25326231] (Western Blot, Human)
• Kong MY, Gaggar A, Li Y Matrix metalloproteinase activity in pediatric acute lung injury. Int J Med Sci, 2008-12-16;6(1):9-17. 2008-12-16 [PMID: 19159011] (Western Blot, Human)
• Schnickmann S, Camacho-Trullio D, Bissinger M, Eils R, Angel P, Schirmacher P, Szabowski A, Breuhahn K AP-1-controlled hepatocyte growth factor activation promotes keratinocyte migration via CEACAM1 and urokinase plasminogen activator/urokinase plasminogen receptor. J. Invest. Dermatol., 2008-11-20;129(5):1140-8. 2008-11-20 [PMID: 19020551] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MMP10
• Uniprot:
  • P09238()