MLH3 Antibody (CL14096) - Azide and BSA Free

Analysis in human endometrium and skeletal muscle tissues using NBP3-43850 antibody. Corresponding MLH3 RNA-seq data are presented for the same tissues.

Staining in A-431 cell line with Anti-MLH3 monoclonal antibody) .

Staining of human endometrial cancer shows moderate nuclear positivity in tumor cells.

Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.

Staining of human endometrium shows moderate nuclear positivity in glandular cells.

Staining of human ovarian cancer (papillary carcinoma) shows strong nuclear positivity in tumor cells.

Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong nuclear positivity in tumor cells.

Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Analysis in human cell line SCLC-21H.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL14096
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

MLH3 Antibody (CL14096) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse MLH3 Antibody (CL14096) - Azide and BSA Free (NBP3-24597) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of Q9UHC1, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MLH3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For Immunocytochemistry/ Immunofluorescence/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for MLH3 Antibody (CL14096) - Azide and BSA Free

• DNA mismatch repair protein Mlh3
• HNPCC7
• MGC138372
• mutL (E. coli) homolog 3
• mutL homolog 3 (E. coli)
• MutL protein homolog 3

Background

MLH3 is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MLH3