MKK3/MEK3 Antibody (1D10) - Azide and BSA Free

Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of MAP2K3 expression in transfected 293T cell line by MAP2K3 monoclonal antibody (M02), clone 1D10.Lane 1: MAP2K3 transfected lysate (Predicted MW: 39.3 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of monoclonal antibody to MAP2K3 on formalin-fixed paraffin-embedded human dysgerminoma. Antibody concentration 3 ug/ml.

Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Western Blot analysis of MAP2K3 expression in Hela NE ( Cat # L013V3 ).

Immunoprecipitation: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of MAP2K3 transfected lysate using anti-MAP2K3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAP2K3 monoclonal antibody.

Sandwich ELISA: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Detection limit for recombinant GST tagged MAP2K3 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 1D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MKK3/MEK3 Antibody (1D10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAP2K3 (AAH32478, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MESPASSQPASMPQSKGKSKRKKDLRISCMSKPPAPNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEKVRHAQSGTIMAVKRIRATVN
• Localization: Secreted
• Specificity: MAP2K3 - mitogen-activated protein kinase kinase 3
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAP2K3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MKK3/MEK3 Antibody (1D10) - Azide and BSA Free

• MAP kinase kinase 3
• MAP2K3
• MAPK/ERK kinase 3
• MAPKK 3
• MAPKK3PRKMK3dual specificity mitogen-activated protein kinase kinase 3
• MEK3
• MEK3MEK 3
• mitogen activated protein kinase kinase 3
• mitogen-activated protein kinase kinase 3
• MKK3
• MKK3EC 2.7.12.2
• PRKMK3

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MKK3/MEK3 Antibody (H00005606-M02)(1)

Publication using H00005606-M02

• Uemura N, Nakanishi Y, Kato H et al. Antibody-based proteomics for esophageal cancer: Identification of proteins in the nuclear factor-kappaB pathway and mitotic checkpoint. Cancer Sci 2009-09-01 [PMID: 19558549]

Bioinformatics

• Gene Symbol: MAP2K3
• Entrez:
  • 5606(Human)
• OMIM:
  • 602315(Human)
• Uniprot:
  • AAH32478(Human)