Mitochondrial ribosomal protein L11 Antibody

Western Blot: Mitochondrial ribosomal protein L11 Antibody [NBP2-17296] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Mitochondrial ribosomal protein L11 Antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Mitochondrial ribosomal protein L11 Antibody [NBP2-17296] - Paraffin-embedded lung carcinoma. MRPL11 antibody [N1C3] dilution: 1:250.

Western Blot: Mitochondrial ribosomal protein L11 Antibody [NBP2-17296] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Rt, RM
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Mitochondrial ribosomal protein L11 Antibody Summary

• Description: Novus Biologicals Rabbit Mitochondrial ribosomal protein L11 Antibody (NBP2-17296) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Mitochondrial ribosomal protein L11. The exact sequence is proprietary.
• Localization: Mitochondrion
• Predicted Species: Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPL11
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (89%), Bovine (87%), Rat (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Mitochondrial ribosomal protein L11 Antibody

• L11MT
• MGC111024
• mitochondrial ribosomal protein L11
• MRP-L11,39S ribosomal protein L11, mitochondrial

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode different isoforms. Pseudogenes corresponding to this gene are found on chromosomes 5q and 12q. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MRPL11
• Uniprot:
  • Q9Y3B7(Human)