Mind Bomb 1/MIB1 Antibody [Alexa Fluor® 647]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

Mind Bomb 1/MIB1 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Mind Bomb 1/MIB1
  Thr365-Gln461
  Accession # Q86YT6
• Specificity: Detects human, mouse and rat Mind Bomb 1/MIB1 in Western blots and detects recombinant human Mind Bomb 1/MIB1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant MIB2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mind Bomb 1/MIB1 Antibody [Alexa Fluor® 647]

• DAPK-interacting protein 1
• DIP1
• DIP-1FLJ90676
• DKFZp686I0769
• DKFZp761M1710
• E3 ubiquitin-protein ligase MIB1
• EC 6.3.2
• EC 6.3.2.-
• KIAA1323
• MGC129660
• MIB
• MIB1
• Mind Bomb 1
• Mind bomb homolog 1
• mindbomb homolog 1 (Drosophila)
• ubiquitin ligase mind bomb
• ubiquitin ligase protein MIB1
• Zinc finger ZZ type with ankyrin repeat domain protein 2
• ZZANK2
• ZZANK2MGC129659
• ZZZ6

Background

MIB1 (Mind bomb 1; also DIP-1 and ZZANK2) is a 110-115 kDa E3 ubiquitin ligase. It is widely expressed in both embryo and adult, and appears to have a non-redundant role in Notch receptor signaling. Notch signaling is initiated when a transmembrane (TM) Notch ligand (Jagged or Delta) binds to Notch on an adjacent cell. This ligation induces cleavage of the Notch extracellular domain (ECD) with the creation of a Jagged/Delta:NotchECD complex. This complex is now internalized by the Notch ligand-expressing cell. The displacement of the NotchECD from its TM segment promotes further Notch processing, resulting in Notch signaling. The key internalization step by the ligand-expressing cell is mediated by the MIB1 E3 ubiquitin ligase. Human MIB1 is 1006 amino acids (aa) in length. It contains multiple interspersed Ankyrin repeats, MIB domains, and zinc-finger motifs. There is one potential alternative start site at Met84. Over aa 365-461, human MIB1 shares 99% aa sequence identity with mouse MIB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.