Mind Bomb 1/MIB1 Antibody (6A9C9)

Images

 
Western Blot: Mind Bomb 1/MIB1 Antibody (6A9C9) [NBP2-61864] - Analysis using MIB1 mAb against HEK293 (1) and MIB1 (AA: 6-221)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Mind Bomb 1/MIB1 Antibody (6A9C9) [NBP2-61864] - Analysis of Hela cells using MIB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with ...read more
Flow Cytometry: Mind Bomb 1/MIB1 Antibody (6A9C9) [NBP2-61864] - Analysis of Hela cells using MIB1 mouse mAb (green) and negative control (red).
Western Blot: Mind Bomb 1/MIB1 Antibody (6A9C9) [NBP2-61864] - Analysis using MIB1 mAb against human MIB1 (AA: 6-221) recombinant protein. (Expected MW is 50.1 kDa)
Western Blot: Mind Bomb 1/MIB1 Antibody (6A9C9) [NBP2-61864] - Analysis using MIB1 mouse mAb against Hela (1) and COS7 (2) cell lysate.
ELISA: Mind Bomb 1/MIB1 Antibody (6A9C9) [NBP2-61864] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
6A9C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Mind Bomb 1/MIB1 Antibody (6A9C9) Summary

Immunogen
Purified recombinant fragment of human MIB1 (AA: 6-221) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MIB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Mind Bomb 1/MIB1 Antibody (6A9C9)

  • DAPK-interacting protein 1
  • DIP1
  • DIP-1FLJ90676
  • DKFZp686I0769
  • DKFZp761M1710
  • E3 ubiquitin-protein ligase MIB1
  • EC 6.3.2
  • EC 6.3.2.-
  • KIAA1323
  • MGC129660
  • MIB
  • MIB1
  • Mind Bomb 1
  • Mind bomb homolog 1
  • mindbomb homolog 1 (Drosophila)
  • ubiquitin ligase mind bomb
  • ubiquitin ligase protein MIB1
  • Zinc finger ZZ type with ankyrin repeat domain protein 2
  • ZZANK2
  • ZZANK2MGC129659
  • ZZZ6

Background

This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). ;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MIB1
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