Migfilin Antibody (3F8) - Azide and BSA Free

Western Blot: Migfilin Antibody (3F8) [H00054751-M03] - Analysis of FBLIM1 expression in HepG2 (Cat # L019V1).

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Migfilin Antibody (3F8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Migfilin Antibody (3F8) - Azide and BSA Free (H00054751-M03) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: FBLIM1 (NP_060026, 270 a.a. ~ 373 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VTCARCIGDESFALGSQNEVYCLDDFYRKFAPVCSICENPIIPRDGKDAFKIECMGRNFHENCYRCEDCRILLSVEPTDQGCYPLNNHLFCKPCHVKRSAAGCC
• Specificity: FBLIM1 - filamin binding LIM protein 1 (3F8)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FBLIM1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Migfilin Antibody (3F8) - Azide and BSA Free

• CAL
• DKFZp434G171
• FBLP-1FBLP1CSX-associated LIM
• filamin binding LIM protein 1
• filamin-binding LIM protein 1
• filamin-binding LIM protein-1
• MIG2-interacting protein
• migfilin
• mitogen-inducible 2 interacting protein
• Mitogen-inducible 2-interacting protein

Background

This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBLIM1
• Entrez:
  • 54751(Human)
• Uniprot:
  • NP_060026(Human)