MID1IP1 Antibody

Western Blot: MID1IP1 Antibody [NBP1-52076] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MID1IP1 with C-terminal MYC tag probed with NBP1-52076 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with NBP1-52076 (1mg/ml) in Lane B.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MID1IP1 Antibody Summary

• Description: Novus Biologicals Goat MID1IP1 Antibody (NBP1-52076) is a polyclonal antibody validated for use in WB and ELISA. Anti-MID1IP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HTVLSKLTRK corresponding to internal region according to NP_067065.1.
• Epitope: C-HTVLSKLTRK
• Specificity: Reported variants represent identical protein: NP_001092261.1, NP_067065.1, NP_001092260.1
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MID1IP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.2 - 1 ug/ml
• Application Notes: WB: In transfected HEK293 transiently expressing full-length human MID1IP1 (myc and DYKDDDDK tagged), a band of approx. 28 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MID1IP1 Antibody

• FLJ10386
• G12-like
• Gastrulation-specific G12-like protein
• MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish))
• MID1 interacting protein 1 (gastrulation specific G12-like (zebrafish))
• MID1 interacting protein 1 (gastrulation specific G12-like)
• Mid1-interacting G12-like protein
• mid1-interacting protein 1
• MIG12MID1 interacting G12-like protein
• Protein STRAIT11499
• S14R
• Spot 14-R
• Spot 14-related protein
• STRAIT11499
• THRSPL

Background

MID1IP1 is involved in stabilization of microtubules

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MID1IP1 Antibody (NBP1-52076)(1)

Publication using NBP1-52076

• Berti C, Fontanella B, Ferrentino R, Meroni G. Mig12, a novel Opitz syndrome gene product partner, is expressed in the embryonic ventral midline and co-operates with Mid1 to bundle and stabilize microtubules. BMC Cell Biol 2004-02-01 [PMID: 15070402]

Bioinformatics

• Gene Symbol: MID1IP1
• Entrez:
  • 58526(Human)
  • 68041(Mouse)
  • 404280(Rat)
• Uniprot:
  • NP_067065.1(Human)