Recombinant Human MICA His Protein

Bioactivity: Recombinant Human MICA Protein [NBP2-52056] - Human MICA is coated at 10ug/ml (100 ul/well) can bind NKG2D (NBP2-60216) in a Functional ELISA assay.

SDS-Page: Recombinant Human MICA Protein [NBP2-52056] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Discontinued

Recombinant Human MICA His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 24-297 of Human MICA
  
  

  Source: E.coli

  Amino Acid Sequence: MEPHSLRYNL TVLSWDGSVQ SGFLTEVHLD GQPFLRCDRQ KCRAKPQGQW AEDVLGNKTW DRETRDLTGN GKDLRMTLAH IKDQKEGLHS LQEIRVCEIH EDNSTRSSQH FYYDGELFLS QNLETEEWTM PQSSRAQTLA MNVRNFLKED AMKTKTHYHA MHADCLQELR RYLKSGVVLR RTVPPMVNVT RSEASEGNIT VTCRASGFYP WNITLSWRQD GVSLSHDTQQ WGDVLPDGNG TYQTWVATRI CQGEEQRFTC YMEHSGNHST HPVPSLEHHH HHH

• Details of Functionality: Human MICA when coated at 10ug/ml (100 ul/well) can bind NKG2D/CD314 (NBP2-60216) in a Functional ELISA assay.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MICA
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 32.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% Glycerol
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human MICA His Protein

• FLJ60820
• MGC111087
• MICA
• PERB11.1

Background

MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MICA