Recombinant Human MICA His Protein

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free

Recombinant Human MICA His Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 320 amino acids corresponding to MICA with a His tagSource: E. coli

  Amino Acid Sequence: MSYYHHHHHH DYDIPTTENL YFQGAMDPEF EPHSLRYNLT VLSWDGSVQS GFLAEVHLDG QPFLRYDRQK CRAKPQGQWA EDVLGNKTWD RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ NLETEEWTVP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLESGVVLRR TVPPMVNVTR SEASEGNITV TCRASSFYPR NIILTWRQDG VSLSHDTQQW GDVLPDGNGT YQTWVATRIC RGEEQRFTCY MEHSGNHSTH PVPSGKVLVL QSHKLGCFGG

• Details of Functionality: MICA Protein is fully biologically active when compared to standard. The specific activity is determined by binding MICA antibody in ELISA.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MICA
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of MICA as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 36.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered concentrated sterile solution in PBS, pH 7.4, and 8 M Urea.
• Preservative: No Preservative
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile dH2O to a conc. of 1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. Make further dilutions in appropriate buffered solutions, with 8M Urea.

Alternate Names for Recombinant Human MICA His Protein

• FLJ60820
• MGC111087
• MICA
• PERB11.1

Background

MIC-A (MHC class I chain-related gene A) is a single-pass type I member protein. It is expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Additionally, MIC-A can be induced by bacterial and viral infections. It shares 85 % amino acid identity with MIC-B and they are distantly related to the MHC class I proteins. Because they possess three extracellular Ig-like domains, but unlike classical MHC class I molecules. They do not form a heterodimer with beta2 microglobulin, but bind as a monomer to a KLRK1/NKG2D that is an activating receptor expressed on NK cells, NKT cells, gammadelta T cells, and CD8+ alphabeta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MIC-A recognition plays an important role in tumor surveillance, viral infections, and autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MICA