MICA/B Antibody (6D4) [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Applications: Flow, IHC, IP
• Clone: 6D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

MICA/B Antibody (6D4) [Alexa Fluor™ Plus 594] Summary

• Immunogen: Transfected C1R cells expressing MICA
• Specificity: Recognizes a common epitope on MICA and MICB glycoproteins, transmembrane ligands of NKG2D, and is able to block NKG2D-mediated activation of NK cells and cytotoxic T cells.
• Isotype: IgG2 Alpha
• Clonality: Monoclonal
• Host: Mouse
• Gene: MICA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MICA/B Antibody (6D4) [Alexa Fluor™ Plus 594]

• MHC class I chain-related protein A
• MHC class I chain-related protein B
• MIC
• MICA
• MICA/B
• MICB
• PERB11
• PERB11.1
• PERB11.2

Background

The MIC gene is located in MHC class I region. MICA/B are 65-75 kD stress-inducible glycoproteins with highly polymorphic. They are MHC class I-like transmembrane molecules that do not associate beta2-microglobulin and do not present peptides. MICA and MICB share 85% identify, and are mainly expressed on Intestinal epithelial cells, epithelial tumor cells, endothelial cells, fibroblasts, and IFN- alpha-stimulated dendritic cells. MIC molecules bind NKG2D, an activating receptor, and induce activation of NK cells, and subset of CD8+ alpha/ beta T cells and gamma/ delta T cells, as well as suppression of T cell proliferation. MICA/B recognition is involved in the regulation of tumor surveillance, viral infection and autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MICA