Recombinant Human MIA Protein

SDS-Page: Recombinant Human MIA Protein [NBP2-61365]

• Reactivity: Hu
• Applications: Bioactivity
• Concentration: LYOPH

Recombinant Human MIA Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 107 amino acids corresponding to MIA Source: E. coli

  Uniprot ID: Q16674

  Amino Acid Sequence: GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ

• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 ug/ml, corresponding to a specific activity of > 200 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MIA
• Purity: >98%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of MIA as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 12.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: 5% Trehalose
• Concentration: LYOPH
• Purity: >98%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human MIA Protein

• CD-RAP
• Melanoma inhibitory activity protein
• melanoma inhibitory activity
• melanoma-derived growth regulatory protein
• MIA

Background

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, includinggliomas

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MIA