MHC class II (I-A/I-E) Antibody (M5/114.15.2) [DyLight 550]

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC, IP
• Clone: M5/114.15.2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: DyLight 550

MHC class II (I-A/I-E) Antibody (M5/114.15.2) [DyLight 550] Summary

• Immunogen: The immunogen for this antibody was MHC Class II.
• Specificity: The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: H2-Ea
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Human reported in scientific literature (PMID:29990503).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [DyLight 550]

• E-alpha-f;H-2 class II histocompatibility antigen, E-K alpha chain;H-2 class II histocompatibility antigen, E-U alpha chain;H-2E;H2-Ea;H-2Ea;H2-Ea-ps;I;Ia;Ia3;Ia-3;I-E;I-Ealpha;MHC-H2-Ea
• H2-Ea
• MHC class II (I-A/I-E)
• MHC class II IA/IE

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2-Ea