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MHC class II (I-A/I-E) Antibody (M5/114.15.2) [DyLight 550]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P
Clone
M5/114.15.2
Clonality
Monoclonal
Host
Rat
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43312AF350
Alexa Fluor 405
NBP1-43312AF405
Alexa Fluor 488
NBP1-43312AF488
Alexa Fluor 532
NBP1-43312AF532
Alexa Fluor 594
NBP1-43312AF594
Alexa Fluor 647
NBP1-43312AF647
Alexa Fluor 700
NBP1-43312AF700
Alexa Fluor 750
NBP1-43312AF750
Allophycocyanin
NBP1-42997-0.1mg
Allophycocyanin
NBP1-43312APC
Allophycocyanin/Cy7
NBP1-43312APCCY7
Biotin
NBP1-43312B
Biotin
NBP1-43552-0.05mg
Biotin
NBP1-43552-0.5mg
DyLight 350
NBP1-43312UV
DyLight 405
NBP1-43312V
DyLight 488
NBP1-43312G
DyLight 594
NBP1-43312DL594
DyLight 650
NBP1-43312C
DyLight 680
NBP1-43312FR
DyLight 755
NBP1-43312IR
FITC
NBP1-43950-0.5mg
FITC
NBP1-43950-0.05mg
FITC
NBP1-43312F
HRP
NBP1-43312H
Janelia Fluor 549
NBP1-43312JF549
Janelia Fluor 646
NBP1-43312JF646
PE
NBP1-43767-0.2mg
PE
NBP1-43767-0.1mg
PE
NBP1-43767-0.05mg
PE
NBP1-43312PE
PE/Atto594
NBP1-43312PEATT594
PE/Cy5
NBP1-43070-0.05mg
PE/Cy5
NBP1-43070-0.1mg
PE/Cy5.5
NBP1-43312PECY55
PE/Cy7
NBP1-43312PECY7
PerCP
NBP1-43312PCP
Unconjugated
NBP1-43312-0.1mg
100% Guarantee on every product

MHC class II (I-A/I-E) Antibody (M5/114.15.2) [DyLight 550] Summary

Immunogen
The immunogen for this antibody was MHC Class II.
Specificity
The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
H2-Ea-ps
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Human reported in scientific literature (PMID:29990503).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [DyLight 550]

  • H2-Ea
  • MHC class II (I-A/I-E)
  • MHC class II IA/IE

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Thyroglobulin
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NBP2-34294
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.
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(1 Publication)
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Applications: Flow, IHC, IHC-P
IL-2 [Unconjugated]
202-IL
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
(25 Reviews)
(320 Publications)
Species: Hu
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
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MHC Class I
Antibody (OX18)
NB120-6405
Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P <=0.01). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-11-63), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.
Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
(32 Publications)
Species: Mu, Rt
Applications: EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready, Flow-IC
IFN-gamma
[Unconjugated]
285-IF
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma.
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
(21 Reviews)
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4-1BB
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AF2295
Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human 4‑1BB Ligand/TNFSF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2295) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for 4‑1BB Ligand/TNFSF9 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
4‑1BB Ligand/TNFSF9 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human 4‑1BB Ligand/TNFSF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2295) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(2 Publications)
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Applications: WB, Flow, IHC, CyTOF-ready
DPEP1 Antibody
NBP1-86087
Western Blot: DPEP1 Antibody [NBP1-86087] - Analysis in control (vector only transfected HEK293T lysate) and DPEP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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(1 Publication)
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CD4 Antibody
NBP1-19371
Immunohistochemistry: CD4 Antibody [NBP1-19371] - Increased CD3+ and CD4+ T-cell occurrence in the brainstem of SHR-72 transgenic rat model for tauopathies. (A-D) Immunofluorescence staining showed CD4+ T-cells in SHR-72 transgenic animals. (E- H) Immunofluorescence staining showed more perivascular than brain parenchyma infiltrating CD4+ T-cells in SHR-72 transgenic animals. PLoS One. 2019 May 23;14(5):e0217216. doi: 10.1371/journal.pone.0217216.
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(33 Publications)
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HLA DPA Antibody
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Immunohistochemistry-Paraffin: SLC26A3 Antibody [NBP1-84450] - Staining of human small intestine shows moderate to strong positivity in apical membrane in glandular cells.
Immunohistochemistry-Paraffin: SLC26A3 Antibody [NBP1-84450] - Staining of human colon shows moderate positivity in glandular cells of apical membrane.
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[Unconjugated]
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Western Blot: Ovalbumin Antibody [NB600-922] - Western blot detection of Ovalbumin from hen egg white using NB600-922 at 1:10,000. The blot was blocked ON at 4 degrees C in 3% BSA. The secondary antibody used was a DyLight 649 Conjugated Goat anti-rabbit IgG (H&L) diluted 1:20,000 and incubated 1 hr RT in blocking buffer for fluorescent western blotting.
Western Blot: Ovalbumin Antibody [NB600-922] - Analysis using the HRP conjugate of NB600-922. Detection of Lane 1: Ovalbumin (Hen Egg). Load: 50 ng per lane. Dilution: 1:1,000. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.
(7 Publications)
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Applications: WB, ELISA, IHC, IHC-P, IP, Single-Cell Western

Publications for MHC class II (I-A/I-E) Antibody (NBP1-43312R) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-43312AF350
Alexa Fluor 405 NBP1-43312AF405
Alexa Fluor 488 NBP1-43312AF488
Alexa Fluor 532 NBP1-43312AF532
Alexa Fluor 594 NBP1-43312AF594
Alexa Fluor 647 NBP1-43312AF647
Alexa Fluor 700 NBP1-43312AF700
Alexa Fluor 750 NBP1-43312AF750
Allophycocyanin NBP1-42997
Allophycocyanin NBP1-43312APC
Allophycocyanin/Cy7 NBP1-43312APCCY7
Biotin NBP1-43312B
Biotin NBP1-43552
Biotin NBP1-43552
DyLight 350 NBP1-43312UV
DyLight 405 NBP1-43312V
DyLight 488 NBP1-43312G
DyLight 550 NBP1-43312R
DyLight 594 NBP1-43312DL594
DyLight 650 NBP1-43312C
DyLight 680 NBP1-43312FR
DyLight 755 NBP1-43312IR
FITC NBP1-43312F
FITC NBP1-43950
FITC NBP1-43950
HRP NBP1-43312H
Janelia Fluor 549 NBP1-43312JF549
Janelia Fluor 646 NBP1-43312JF646
PE NBP1-43767
PE NBP1-43767
PE NBP1-43767
PE NBP1-43312PE
PE/Atto594 NBP1-43312PEATT594
PE/Cy5 NBP1-43070
PE/Cy5 NBP1-43070
PE/Cy5.5 NBP1-43312PECY55
PE/Cy7 NBP1-43312PECY7
PerCP NBP1-43312PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol H2-Ea-ps