MHC class II (I-A/I-E) Antibody (M5/114.15.2) [Allophycocyanin]

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [Allophycocyanin] [NBP1-42997] - Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC and 0.015 micrograms of Rat IgG2b ? Isotype Control APC (left) or 0.015 micrograms of Anti-Mouse MHC Class II (I-A/I-E) APC (right). Cells in the lymphocyte gate were used for analysis.

Product Discontinued

MHC class II (I-A/I-E) Antibody (M5/114.15.2) [Allophycocyanin] Summary

• Immunogen: The immunogen for this antibody was MHC Class II.
• Specificity: The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: H2-Ea
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

Use in Human reported in scientific literature (PMID:29990503).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [Allophycocyanin]

• E-alpha-f;H-2 class II histocompatibility antigen, E-K alpha chain;H-2 class II histocompatibility antigen, E-U alpha chain;H-2E;H2-Ea;H-2Ea;H2-Ea-ps;I;Ia;Ia3;Ia-3;I-E;I-Ealpha;MHC-H2-Ea
• H2-Ea
• MHC class II (I-A/I-E)
• MHC class II IA/IE

Background

The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2-Ea