MHC Class II Antibody

Product Discontinued

MHC Class II Antibody Summary

• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CIITA. The immunogen is located within amino acids 120 - 170 of CIITA.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HLA-DMA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1-2 ug/ml
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23918395)
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MHC Class II Antibody

• class II histocompatibility antigen, M alpha chain
• D6S222E
• DMA
• HLA class II histocompatibility antigen, DM alpha chain
• major histocompatibility complex, class II, DM alpha
• MHC class II antigen DMA
• Really interesting new gene 6 protein
• RING6HLADM

Background

CIITA contains an acidic transcriptional activation domain, four LRRs (leucine-rich repeats) and a GTP binding domain. It is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. CIITA also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MHC Class II Antibody (NBP1-76296)(3)

Publications using NBP1-76296

• Abu-El-Rub E Effect of hypoxic/ischemic environment on the immunoprivilege of allogeneic mesenchymal stem cells Thesis 2020-01-01 (WB, Rat, Human)
• Hassan R, Alley E, Kindler H et al. Live-Attenuated, Listeria monocytogenes Expressing Mesothelin (CRS-207) with Chemotherapy for Treatment of Malignant Pleural Mesothelioma Clin. Cancer Res. 2019-07-01 [PMID: 31263030] (IF/IHC, Human)
• Awojoodu AO, Ogle ME, Sefcik LS et al. Sphingosine 1-phosphate receptor 3 regulates recruitment of anti-inflammatory monocytes to microvessels during implant arteriogenesis. Proc Natl Acad Sci U S A. 2013-08-20 [PMID: 23918395] (IHC-P, ICC/IF, Mouse)

Reviews for MHC Class II Antibody (NBP1-76296) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Rat.

reviewed by: Ejlal Abuelrub WB Rat 03/03/2015

Summary

• Application: Western Blot
• Species: Rat

reviewed by: Hayley Derricott IHC-P Human 03/20/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human tonsil
• Species: Human
• Lot: 5979-1003

FAQs for MHC Class II Antibody (NBP1-76296). (Showing 1 - 1 of 1 FAQs).

1. I would like to ask you about the specificity of MHC Class II Antibody (Cat. # NBP1-76296). As this antibody is designed to recognize a sequence of CIITA according to the data sheet, can we discuss that it would be comparable to MHC Class II expressions w
   • NBP1-76296 has been validated in IHC-Pariffin sections for human, mouse, and rat. Depending on the tissue used and the CIITA protein expression in that tissue, you can expect this antibody to recognize the target in IHC-P tissue sections.

Bioinformatics

• Gene Symbol: HLA-DMA
• Entrez:
  • 4261(Human)
• Uniprot:
  • P33076(Human)