AS3MT Antibody

Immunohistochemistry-Paraffin: AS3MT Antibody [NB100-1131] - (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, ArHa
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AS3MT Antibody Summary

• Description: Novus Biologicals Goat AS3MT Antibody (NB100-1131) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-AS3MT Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence AALRDAEIQKDVQ-C corresponding to N-Terminus according to NP_065733.2.
• Epitope: AALRDAEIQKDVQ-C
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AS3MT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1:100 - 1:2000
• Application Notes: IHC-P: Human kidney shows cytoplasm staining of epithelial cells with some emphasis on the luminal side of the DCT. WB reactivity reported in (PMID: 21537954).

Reactivity Notes

Armenian Hamster reactivity reported in scientific literature (PMID: 21537954).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AS3MT Antibody

• Arsenic (+3 Oxidation State) Methyltransferase
• Arsenic Methyltransferase
• arsenite methyltransferase
• AS3MT
• CYT19
• CYT19methyltransferase cyt19
• EC 2.1.1.137
• Methylarsonite Methyltransferase
• Methyltransferase Cyt19
• S-Adenosyl-L-Methionine:Arsenic(III) Methyltransfe
• S-adenosyl-L-methionine:arsenic(III) methyltransferase
• S-Adenosylmethionine:Arsenic (III) Methyltransfera
• S-adenosylmethionine:arsenic (III) methyltransferase

Background

AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and mayplay a role in arsenic metabolism (Lin et al., 2002 (PubMed 11790780)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AS3MT Antibody (NB100-1131)(2)

Publications using NB100-1131

• Watanabe T, Ohta Y, Mizumura A et al. Analysis of arsenic metabolites in HepG2 and AS3MT-transfected cells. Arch Toxicol85(6):577-88. 2011-06-01 [PMID: 21537954] (WB, Armenian Hamster)
• Lin S, Shi Q, Nix FB et al. A novel S-adenosyl-L-methionine:arsenic(III) methyltransferase from rat liver cytosol. J Biol Chem 2002 Mar 29 [PMID: 11790780]

Bioinformatics

• Gene Symbol: AS3MT
• Entrez:
  • 57412(Human)
• Uniprot:
  • NP_065733.2(Human)