Mouse MHC Class I ELISA Kit (Chemiluminescence)

Standard Curve Reference.

Samples were spiked with high concentrations of Mouse MHC Class I, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 3.13 - 200 pg/mL
• Sensitivity: 1.88 pg/mL

Mouse MHC Class I ELISA Kit (Chemiluminescence) Summary

• Description: Assay Length: 3 h 30 min
• Specificity: This kit recognizes Mouse MHC Class I in samples. No significant cross-reactivity or interference between Mouse MHC Class I and analogues was observed
• Standard Curve Range: 3.13 - 200 pg/mL
• Sensitivity: 1.88 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10
• Intra-Assay: CV% < 10
• Spike Recovery: 80 - 120%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: HLA-E

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse MHC Class I ELISA Kit (Chemiluminescence)

• DKFZp686P19218
• EA1.2
• EA2.1
• HLA class I histocompatibility antigen, alpha chain E
• HLA class I histocompatibility antigen, E alpha chain
• HLA-6.2
• HLAE
• lymphocyte antigen
• major histocompatibility complex, class I, E
• MHC class I antigen E
• MHC HLA-E alpha-1
• MHC HLA-E alpha-2.1
• MHC
• QA1

Background

The antigenic agglomerate major histocompatibility complex (MHC) class I molecules present antigenic peptides to CD8-positive T cells. It is now recognized that the products of the MHC genes are antigen-presenting molecules (APM) designed for the presentation of antigen fragments (peptides) to the T-cell receptor, thus participating in the immune response (1). Recently shown is a mechanism of antigen acquisition for cross-presentation that couples the antigen presentation system of two adjacent cells and is lost in most tumours: gap-junction-mediated intercellular peptide coupling for presentation by bystander MHC class I molecules and transfer to professional antigen presenting cells for cross-priming (2). The mammalian vomeronasal organ detects social information about gender, status, and individuality. It has been shown that small peptides that serve as ligands for major histocompatibility complex (MHC) class I molecules function also as sensory stimuli for a subset of vomeronasal sensory neurons located in the basal Gao- and V2R receptor-expressing zone of the vomeronasal epithelium (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for MHC Class I ELISA Kit (NBP3-49832). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HLA-E