MHC Class I Antibody (F21-2) [FITC]

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Flow Cytometry: MHC Class I Antibody (F21-2) [FITC] [NBP1-28305] - 1 ug/10^6 Chicken peripheral blood mononuclear cells were double-stained with mouse anti-chicken MHC Class I-FITC and mouse anti-chicken IgM-PE. Small ...read more

Product Details

Summary
Reactivity Av, Ch, TrSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IP
Clone
F21-2
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

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Catalog# & Conjugate Size Price

MHC Class I Antibody (F21-2) [FITC] Summary

Immunogen
A monomorphic epitope on avian MHC Class I molecules (Mr approx. 68 kDa)
Specificity
A monomorphic epitope on avian MHC Class I molecules (Mr approx. 68 kDa)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HLA-E
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Chicken and Turkey

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for MHC Class I Antibody (F21-2) [FITC]

  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • QA1

Background

Like their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens) consist of a highly polymorphic alpha-chain (Mr approx. 44 kDa) non-covalently bound to the invariant beta2-microglobulin subunit (Mr approx. 12 kDa). MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (1-3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MHC Class I Antibody (NBP1-28305). (Showing 1 - 1 of 1 FAQ).

  1. Has this product, NBP1-28305, been tested for cross reactivity with human MHC class I or any other human cell surface markers?
    • NBP1-28305 is not tested for any cross-reactivity against human. For human MHC class I, I would recommend our product NB100-64212.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-28306

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Bioinformatics Tool for MHC Class I Antibody (NBP1-28305)

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Blogs on MHC Class I.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

Major Histocompatibility Complex (MHC) Class I
The products of MHC genes are antigen-presenting molecules (APMs) designed for antigen fragment (peptide) presentation to the T-cell receptor. In particular, MHC Class I molecules play a key role in the immune system by presenting endogenously synthes...  Read full blog post.

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Bioinformatics

Gene Symbol HLA-E