MHC Class I Antibody (2G5) [DyLight 755]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, GP, Ha, ShSpecies Glossary
Applications Flow, IP
Clone
2G5
Clonality
Monoclonal
Host
Mouse

MHC Class I Antibody (2G5) [DyLight 755] Summary

Immunogen
Purified H-2Kb and H-2Db MHC-I molecules.
Specificity
NB100-65938 recognizes a monomorphic epitope on the mouse MHC class I antigen. The epitope recognized by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this antibody may recognize a polymorphic epitope of canine MHC class 1.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HLA-E
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 755
Format
DyLight 755 (A=754, E=776)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunoprecipitation
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 10^6 cells in 10 ul.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for MHC Class I Antibody (2G5) [DyLight 755]

  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • QA1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for MHC Class I Antibody (NB100-65938IR) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-65938APC
DyLight 350 Labeled NB100-65938UV
DyLight 405 Labeled NB100-65938V
DyLight 405LS Labeled NB100-65938V3
DyLight 488 Labeled NB100-65938G
DyLight 680 Labeled NB100-65938FR
PE Labeled NB100-65938PE
PerCP Labeled NB100-65938PCP

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Blogs on MHC Class I.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

Major Histocompatibility Complex (MHC) Class I
The products of MHC genes are antigen-presenting molecules (APMs) designed for antigen fragment (peptide) presentation to the T-cell receptor. In particular, MHC Class I molecules play a key role in the immune system by presenting endogenously synthes...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol HLA-E

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