mGluR2/3 Antibody

Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Western blot of 10 ug of rat hippocampal lysate showing the specific immunolabeling of the ~110k monomer and the ~220k dimer of mGluR2 and mGluR3.

Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Western blot of 10 ug of HEK 293 cells expression. As shown in the autoradiograph, the mGluR2/3 antibody recognizes both the ~110k monomer and the ~220k dimer of mGluR2 (Lane A) and mGluR3 (Lane B). Also shown is the specific immunolabeling of the monomer

Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Specificity of mGluR2/3 (NB 300-124) in rat tissue lysate.

Product Discontinued

mGluR2/3 Antibody Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
• Localization: Integral membrane protein
• Specificity: Specific for the ~110k monomer and the ~220k dimer of mGluR2 and mGluR3. Immunolabeling is blocked by preadsorption of antibody with the peptide used as antigen to generate the antibody.
• Predicted Species: Human (100%), Canine (100%), Primate (100%), Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:500
  • Western Blot 1:1000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 17099896)
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 17099896).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for mGluR2/3 Antibody

• GLUR2
• glutamate metabotropic receptor 2
• glutamate receptor, metabotropic 2
• GPRC1Bmetabotropic glutamate receptor 2
• mGlu2
• mGluR2
• mGluR2/3
• MGLUR2glutamate receptor homolog

Background

Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on the ligand-gated receptor channels-termed NMDA, AMPA and kainate receptors that are involved in the fast excitatory synaptic transmission. Recently, glutamate has been shown to regulate enzymes producing second messengers via specific receptors coupled to G-proteins. These receptors are called metabotropic glutamate receptors. In expression systems, Group-I receptors, stimulate phospholipase C as revealed by an increase in phosphoinositide turnover, and Ca2+ release from internal stores. Group-II and -III receptors are coupled to the inhibition of adenylyl cyclase. The Group-I receptors include mGluR1a and mGluR5. The Group-II receptors in include mGluR2 and mGluR3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mGluR2/3 Antibody (NB300-124)(5)

Publications using NB300-124

• Cisani F, Roggeri A, Olivero G et al. Acute Low Dose of Trazodone Recovers Glutamate Release Efficiency and mGlu2/3 Autoreceptor Impairments in the Spinal Cord of Rats Suffering From Chronic Sciatic Ligation Front. Pharmacol. 2020-07-17 [PMID: 32765286] (WB, Rat)
• AR Malik, K Szydlowska, K Nizinska, A Asaro, EA van Vliet, O Popp, G Dittmar, R Fritsche-G, JA Kirwan, A Nykjaer, K Lukasiuk, E Aronica, TE Willnow SorCS2 Controls Functional Expression of Amino Acid Transporter EAAT3 and Protects Neurons from Oxidative Stress and Epilepsy-Induced Pathology Cell Rep. 2019-01-01 [PMID: 30840898] (WB, Rat)
• Olivero G, Grilli M, Vergassola M et al. 5-HT2A-mGlu2/3 receptor complex in rat spinal cord glutamatergic nerve endings: A 5-HT2Ato mGlu2/3 signalling to amplify presynaptic mechanism of auto-control of glutamate exocytosis Neuropharmacology 2018-05-01 [PMID: 29499271] (WB, Rat)
• Simat M, Parpan F, Fritschy JM. Heterogeneity of glycinergic and gabaergic interneurons in the granule cell layer of mouse cerebellum. J Comp Neurol;500(1):71-83. 2007-01-01 [PMID: 17099896] (ICC/IF, Mouse)
• Sanabria V, Perosa S, Bittencourt S Et Al. Immunofluorescence co-localization of progesterone receptor with glutamatergic interneurons in the ca3 hippocampal region of an ovariectomized female proechimys guyannensis Epilepsy & Behavior 2021-08-01 (ICC/IF)

Bioinformatics

• Gene Symbol: GRM2
• Entrez:
  • 29627(Rat)
• Uniprot:
  • P31421(Rat)