MgcRacGAP/RACGAP1 Antibody

Images

 
Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7.5% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Paraformaldehyde-fixed A431, using RACGAP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha ...read more
Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Paraffin-embedded Hepatoma.
Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Sample (50 ug of whole cell lysate) A: mouse heart 7.5% SDS PAGE diluted at 1:1000
Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Paraffin-embedded H1299 xenograft.

Product Details

Summary
Reactivity Hu, Mu, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MgcRacGAP/RACGAP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human RACGAP1. The exact sequence is proprietary.
Localization
Nucleus, Cytoplasm, Spindle, Acrosome
Predicted Species
Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RACGAP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rat (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MgcRacGAP/RACGAP1 Antibody

  • GTPase activating protein
  • HsCYK-4
  • ID-GAP
  • KIAA1478
  • Male germ cell RacGap
  • MgcRacGAP
  • MgcRacGAPrac GTPase-activating protein 1
  • Rac GTPase activating protein 1
  • RACGAP1

Background

Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO, RAC and CDC42. GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RACGAP1
Entrez