MgcRacGAP/RACGAP1 Antibody - BSA Free

Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7.5% SDS PAGE diluted at 1:1000

Immunocytochemistry/Immunofluorescence: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Paraformaldehyde-fixed A431, using RACGAP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Human hepatoma, using RACGAP1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Sample (50 ug of whole cell lysate) A: mouse heart 7.5% SDS PAGE diluted at 1:1000

Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - H1299 xenograft , using RACGAP1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MgcRacGAP/RACGAP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MgcRacGAP/RACGAP1 Antibody - BSA Free (NBP1-33455) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-MgcRacGAP/RACGAP1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human MgcRacGAP/RACGAP1. The exact sequence is proprietary.
• Localization: Nucleus, Cytoplasm, Spindle, Acrosome
• Predicted Species: Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RACGAP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MgcRacGAP/RACGAP1 Antibody - BSA Free

• GTPase activating protein
• HsCYK-4
• ID-GAP
• KIAA1478
• Male germ cell RacGap
• MgcRacGAP
• MgcRacGAPrac GTPase-activating protein 1
• Rac GTPase activating protein 1
• RACGAP1

Background

Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO, RAC and CDC42. GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MgcRacGAP/RACGAP1 Antibody (NBP1-33455)(3)

Publications using NBP1-33455

• Chen H, Yang W, Li Y, Ji Z PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling Cell death & disease 2023-07-15 [PMID: 37454211] (IHC-Fr, Human)
• Li Q, Wang M, Hu Y et al. MYBL2 disrupts the Hippo-YAP pathway and confers castration resistance and metastatic potential in prostate cancer Theranostics 2021-03-31 [PMID: 33897882] (WB, IF/IHC, Human)
• Yang XM, Cao XY, He P et al. Overexpression of Rac GTPase activating Protein 1 Contributes to Proliferation of Cancer Cells by Reducing Hippo Signaling to Promote Cytokinesis. Gastroenterology. 2018-07-12 [PMID: 30009820] (WB, Human)

Bioinformatics

• Gene Symbol: RACGAP1