MgcRacGAP/RACGAP1 Antibodies
We offer MgcRacGAP/RACGAP1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each MgcRacGAP/RACGAP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our MgcRacGAP/RACGAP1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the MgcRacGAP/RACGAP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our MgcRacGAP/RACGAP1 polyclonal antibodies and browse our MgcRacGAP/RACGAP1 monoclonal antibody catalog.
Alternate Names for MgcRacGAP/RACGAP1 Antibodies
anti-MgcRacGAP/RACGAP1 antibody, anti-RACGAP1 antibody, anti-GTPase activating protein antibody, anti-HsCYK-4 antibody, anti-ID-GAP antibody, anti-KIAA1478 antibody, anti-Male germ cell RacGap antibody, anti-MGCRACGAP antibody, anti-MgcRacGAPrac GTPase-activating protein 1 antibody, anti-Rac GTPase activating protein 1 antibody
13 Results for "mgcracgap-racgap1" in Primary Antibodies
![Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - RACGAP1 monoclonal antibody (M01), clone 1G6. Analysis of RACGAP1 expression in Jurkat.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Western-Blot-H00029127-M01-img0010.jpg "Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - RACGAP1 monoclonal antibody (M01), clone 1G6. Analysis of RACGAP1 expression in Jurkat.")
![Immunocytochemistry/Immunofluorescence: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - iASPP-dependent localization of midbody markers.a Localization of midbody markers in control, iASPP-depleted, or CEP55-depleted HeLa cells. HeLa cells were transfected with indicated siRNAs. After 48?h, cells were immunostained for midbody markers (green), Beta-tubulin (red), and DNA (blue). b Quantification of proportions of cells from a displaying defects in localization of markers at the midbody Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-018-0561-6) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Immunocytochemistry-Immunofluorescence-H00029127-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Analysis of monoclonal antibody to RACGAP1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Immunohistochemistry-Paraffin-H00029127-M01-img0007.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Western Blot analysis of RACGAP1 expression in K-562 ( Cat # L009V1 ).](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Western-Blot-H00029127-M01-img0003.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - RACGAP1 monoclonal antibody (M01), clone 1G6. Analysis of RACGAP1 expression in 293.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Western-Blot-H00029127-M01-img0009.jpg)
![ELISA: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Detection limit for recombinant GST tagged RACGAP1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-ELISA-H00029127-M01-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - iASPP-dependent localization of midbody markers.a Localization of midbody markers in control, iASPP-depleted, or CEP55-depleted HeLa cells. HeLa cells were transfected with indicated siRNAs. After 48 h, cells were immunostained for midbody markers (green), beta -tubulin (red), & DNA (blue). b Quantification of proportions of cells from a displaying defects in localization of markers at the midbody Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29743530), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00029127-m01_mouse-monoclonal-mgcracgap-racgap1-antibody-1g6-31020241535656.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody [NB100-884] - (10ug protein in RIPA buffer) overexpressing Human RACGAP1 with DYKDDDDK tag probed with EB05315 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/500) in lane C. Mock-transfected HEK293 probed with EB05315 (1mg/ml) in Lane B. Prima](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Western-Blot-NB100-884-img0006.jpg "Western Blot: MgcRacGAP/RACGAP1 Antibody [NB100-884] - (10ug protein in RIPA buffer) overexpressing Human RACGAP1 with DYKDDDDK tag probed with EB05315 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/500) in lane C. Mock-transfected HEK293 probed with EB05315 (1mg/ml) in Lane B. Prima")
![Immunocytochemistry/Immunofluorescence: MgcRacGAP/RACGAP1 Antibody [NB100-884] - MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-884-img0007.jpg)
![Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NB100-884] - (4ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunohistochemistry-Paraffin-NB100-884-img0005.jpg)
![Flow Cytometry: MgcRacGAP/RACGAP1 Antibody [NB100-884] - MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Flow-Cytometry-NB100-884-img0008.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-RACGAP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Western-Blot-NBP2-47374-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-RACGAP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47374-img0004.jpg)
![Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Staining of human Placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47374-img0013.jpg)
![Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47374-img0006.jpg)
![Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47374-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Staining in human testis and endometrium tissues using anti-RACGAP1 antibody. Corresponding RACGAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47374-img0008.jpg)
![Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Analysis in human testis and pancreas tissues using NBP2-47374 antibody. Corresponding RACGAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47374-img0010.jpg)
![Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Staining of human Lymph node shows moderate nuclear positivity in germinal center and non-germinal center cells.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47374-img0011.jpg)
![Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Staining of human Pancreas shows no nuclear positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47374-img0012.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Western-Blot-NBP1-33455-img0015.jpg "Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7.5% SDS PAGE diluted at 1:1000")
![Immunocytochemistry/Immunofluorescence: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Paraformaldehyde-fixed A431, using RACGAP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33455-img0010.jpg)
![Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Human hepatoma, using RACGAP1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-33455-img0013.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - Sample (50 ug of whole cell lysate) A: mouse heart 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Western-Blot-NBP1-33455-img0014.jpg)
![Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP1-33455] - H1299 xenograft , using RACGAP1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-33455-img0012.jpg)
![Western Blot: RACGAP1 Antibody [NBP2-20049] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RACGAP1-Antibody-Western-Blot-NBP2-20049-img0001.jpg "Western Blot: RACGAP1 Antibody [NBP2-20049] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: RACGAP1 Antibody [NBP2-20049] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RACGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20049-img0003.jpg)
![Immunohistochemistry-Paraffin: RACGAP1 Antibody [NBP2-20049] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RACGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-20049-img0002.jpg)
![Immunohistochemistry: MgcRacGAP/RACGAP1 Antibody [NBP2-20049] - Immunohistochemical analysis (whole mount) of zebrafish embryo, using racgap1antibody (NBP2-20049) at 1:200 dilution.](http://images.novusbio.com/fullsize/nbp2-20049_rabbit-polyclonal-mgcracgap-racgap1-antibody-91020242021943.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody (10F7U6) [NBP3-15700] - Western blot analysis of extracts of various cell lines, using MgcRacGAP/RACGAP1 antibody (NBP3-15700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-10F7U6-Western-Blot-NBP3-15700-img0001.jpg "Western Blot: MgcRacGAP/RACGAP1 Antibody (10F7U6) [NBP3-15700] - Western blot analysis of extracts of various cell lines, using MgcRacGAP/RACGAP1 antibody (NBP3-15700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: MgcRacGAP/RACGAP1 Antibody (10F7U6) [NBP3-15700] - Western blot analysis of extracts of PC-12 cells, using MgcRacGAP/RACGAP1 antibody (NBP3-15700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-10F7U6-Western-Blot-NBP3-15700-img0002.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody (SR2170) [NBP3-22337] - Western blot analysis using NBP3-22337 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22337_rabbit-mgcracgap-racgap1-mab-sr2170-672023106273.jpg "Western Blot: MgcRacGAP/RACGAP1 Antibody (SR2170) [NBP3-22337] - Western blot analysis using NBP3-22337 in A431 cell lysate.")
![Western Blot: MgcRacGAP/RACGAP1 Antibody (JE41-19) [NBP3-32889] - Western blot analysis of MgcRacGAP/RACGAP1 on different lysates with Rabbit anti-MgcRacGAP/RACGAP1 antibody (NBP3-32889) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: 293T cell lysate Lane 4: HeLa cell lysate Lane 5: PC-12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32889) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32889_rabbit-mgcracgap-racgap1-mab-je41-19-28820241244299.jpg "Western Blot: MgcRacGAP/RACGAP1 Antibody (JE41-19) [NBP3-32889] - Western blot analysis of MgcRacGAP/RACGAP1 on different lysates with Rabbit anti-MgcRacGAP/RACGAP1 antibody (NBP3-32889) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: 293T cell lysate Lane 4: HeLa cell lysate Lane 5: PC-12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32889) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP1-47267] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RACGAP1 antibody NBP1-47267 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. RACGAP1 was also immunoprecipitated by rabbit anti-RACGAP1 antibody NBP1-47268, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Western-Blot-NBP1-47267-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP1-47267] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RACGAP1 antibody NBP1-47267 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. RACGAP1 was also immunoprecipitated by rabbit anti-RACGAP1 antibody NBP1-47268, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Immunocytochemistry/Immunofluorescence: MgcRacGAP/RACGAP1 Antibody [NBP2-55429] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55429-img0001.jpg "Immunocytochemistry/Immunofluorescence: MgcRacGAP/RACGAP1 Antibody [NBP2-55429] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Western Blot: MgcRacGAP/RACGAP1 Antibody (4B7) [H00029127-M03] - Detection against Immunogen (37.73 KDa) .](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-4B7-Western-Blot-H00029127-M03-img0001.jpg "Western Blot: MgcRacGAP/RACGAP1 Antibody (4B7) [H00029127-M03] - Detection against Immunogen (37.73 KDa) .")