MgcRacGAP/RACGAP1 Antibody (JE41-19)

Western Blot: MgcRacGAP/RACGAP1 Antibody (JE41-19) [NBP3-32889] - Western blot analysis of MgcRacGAP/RACGAP1 on different lysates with Rabbit anti-MgcRacGAP/RACGAP1 antibody (NBP3-32889) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: 293T cell lysate Lane 4: HeLa cell lysate Lane 5: PC-12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32889) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: JE41-19
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MgcRacGAP/RACGAP1 Antibody (JE41-19) Summary

• Description: Novus Biologicals Rabbit MgcRacGAP/RACGAP1 Antibody (JE41-19) (NBP3-32889) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide. (Uniprot: Q9H0H5)
• Localization: Nucleus, Cytoplasm, cytoskeleton, spindle, Cytoplasmic vesicle, secretory vesicle, acrosome, Cleavage furrow, Midbody, Midbody ring, Cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RACGAP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MgcRacGAP/RACGAP1 Antibody (JE41-19)

• GTPase activating protein
• HsCYK-4
• ID-GAP
• KIAA1478
• Male germ cell RacGap
• MgcRacGAP
• MgcRacGAPrac GTPase-activating protein 1
• Rac GTPase activating protein 1
• RACGAP1

Background

Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RACGAP1