MGC20410 Antibody (PCRP-BATF2-2B9) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: ELISA, Flow, IHC, IP
• Clone: PCRP-BATF2-2B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

MGC20410 Antibody (PCRP-BATF2-2B9) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Recombinant fragment (around aa5-140) of human MGC20410 (exact sequence is proprietary)
• Localization: Nucleus.
• Specificity: This antibody recognizes a transcription factor involved in differentiation of CD8+ thymic dendritic cells MGC20410 has been implicated in breast cancer, malignant glioma and metastatis melanoma progression
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BATF2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for MGC20410 Antibody (PCRP-BATF2-2B9) [CoraFluor™ 1]

• basic leucine zipper transcription factor, ATF-like 2
• basic leucine zipper transcriptional factor ATF-like 2
• B-ATF-2
• MGC20410
• SARI
• suppressor of AP-1 regulated by IFN

Background

BATF2 (Basic leucine zipper transcriptional factor ATF-like 2) is a 274 amino acid member of the AP-1 transcription factor family. It controls the differentiation of lineage-specific cells in the immune system. It participates in differentiating CD8+ thymic conventional dendritic cells and acts by forming a heterodimer with JUN family proteins. They then recognize and bind the DNA sequence 5'-TGA(CG)TCA-3'. It acts as a tumor suppressor by interfering with AP-1 regulated activation of CYR61/CCN1 transcription and its downstream signaling. CYR61/CCN1 signaling induces anchorage-independent growth and invasion in several cancer types, such as breast cancer, malignant glioma and metastatic melanoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BATF2