MGAT1 Antibody - Azide and BSA Free

Western Blot: MGAT1 Antibody [H00004245-B01P] - Analysis of MGAT1 expression in transfected 293T cell line by MGAT1 polyclonal antibody. Lane 1: MGAT1 transfected lysate(48.95 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MGAT1 Antibody [H00004245-B01P] - Analysis of purified antibody to MGAT1 on HeLa cell. (antibody concentration 10 ug/ml)

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MGAT1 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: MGAT1 (NP_002397.1, 1 a.a. - 445 a.a.) full-length human protein. MLKKQSAGLVLWGAILFVAWNALLLLFFWTRPAPGRPPSVSALDGDPASLTREVIRLAQDAEVELERQRGLLQQIGDALSSQRGRVPTAAPPAQPRVPVTPAPAVIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDCGHEETAQAIASYGSAVTHIRQPDLSSIAVPPDHRKFQGYYKIARHYRWALGQVFRQFRFPAAVVVEDDLEVAPDFFEYFRATYPLLKADPSLWCVSAWNDNGKEQMVDASRPELLYRTDFFPGLGWLLLAELWAELEPKWPKAFWDDWMRRPEQRQGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQFVHFTQLDLSYLQREAYDRDFLARVYGAPQLQVEKVRTNDRKELGEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAPPPTWEGYDPSWN
• Specificity: MGAT1 - mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: MGAT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MGAT1 Antibody - Azide and BSA Free

• alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
• EC 2.4.1.101
• GGNT1
• GlcNAc-T I
• GlcNac-TI
• GLCT1
• GLYT1GLCNAC-TI
• GNT-1
• GNT-I
• mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
• MGAT
• MGAT1
• N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I

Background

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MGAT1
• Entrez:
  • 4245(Human)
• Uniprot:
  • NP_002397.1(Human)