Human Methionine Aminopeptidase 2/METAP2 - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human Methionine Aminopeptidase 2/METAP2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39861] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates and other biological fluids
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.058 ng/mL (example only; lot dependent)

Human Methionine Aminopeptidase 2/METAP2 - Ready-To-Use ELISA Kit (Colorimetric) Summary

• Description: The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
  Assay Length: 3 hours
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.058 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: METAP2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Methionine Aminopeptidase 2/METAP2 - Ready-To-Use ELISA Kit (Colorimetric)

• Amp2
• eIF-2-associated p67 homolog
• Initiation factor 2-associated 67 kDa glycoprotein
• MAP 2
• MAP2
• MetAP 2
• METAP2
• Methionine Aminopeptidase 2
• methionyl aminopeptidase 2
• MNPEP
• p67
• p67EC 3.4.11.18
• p67eIF2
• Peptidase M 2

Background

METAP2 is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Methionine Aminopeptidase 2/METAP2 ELISA Kit (NBP3-39861). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: METAP2