Mesp1 Antibody (939826) [Unconjugated]

Images

 
Mouse ES cells transfected with human MESP1 were stained with Mouse Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB92193, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed ...read more
Western blot shows lysates of Mouse ES cells transfected with human MESP1 untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MESP1 ...read more
Western blot shows lysates of H1 human embryonic stem cells untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with Mouse Anti-Human MESP1 Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
939826
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Mesp1 Antibody (939826) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human MESP-1
Met1-Gln85
Accession # Q9BRJ9
Specificity
Detects human MESP-1 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mesp1 Antibody (939826) [Unconjugated]

  • bHLHc5
  • bHLHc5MGC10676
  • Class C basic helix-loop-helix protein 5
  • mesoderm posterior 1 homolog (mouse)
  • mesoderm posterior protein 1
  • MESP1

Background

Mesoderm posterior protein 1 (Mesp-1) is a 268 amino acids protein that in humans is encoded by the MESP1 gene. Mesp-1 was first identified in transcripts enriched in the posterior region of the mouse embryo at embryonic day E7 to E7.5. Lineage tracing in mice showed that Mesp-1 represents the earliest marker of cardiac progenitors and directs multipotential cardiovascular cell fates, patterning mesoderm into cardiac, hematopoietic, or skeletal myogenic progenitors in a context-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Mesp1 Antibody (MAB92193) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC92193U
Alexa Fluor 405 IC92193V
Alexa Fluor 488 IC92193G
Alexa Fluor 594 IC92193T
Alexa Fluor 647 IC92193R
Alexa Fluor 700 IC92193N
Alexa Fluor 750 IC92193S

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