Mesp1 Antibody (939826) [Unconjugated]

Mouse ES cells transfected with human MESP1 were stained with Mouse Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB92193, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Western blot shows lysates of Mouse ES cells transfected with human MESP1 untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB92193) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MESP1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Cells provided courtesy of Michael Kyba's lab, University of Minnesota.

Western blot shows lysates of H1 human embryonic stem cells untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with Mouse Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB92193) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MESP1 at approximately 30 kDa (as indicated).Image provided courtesy of Michael Kyba's lab, University of Minnesota.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clone: 939826
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Mesp1 Antibody (939826) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MESP-1
  Met1-Gln85
  Accession # Q9BRJ9
• Specificity: Detects human MESP-1 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mesp1 Antibody (939826) [Unconjugated]

• bHLHc5
• bHLHc5MGC10676
• Class C basic helix-loop-helix protein 5
• mesoderm posterior 1 homolog (mouse)
• mesoderm posterior protein 1
• MESP1

Background

Mesoderm posterior protein 1 (Mesp-1) is a 268 amino acids protein that in humans is encoded by the MESP1 gene. Mesp-1 was first identified in transcripts enriched in the posterior region of the mouse embryo at embryonic day E7 to E7.5. Lineage tracing in mice showed that Mesp-1 represents the earliest marker of cardiac progenitors and directs multipotential cardiovascular cell fates, patterning mesoderm into cardiac, hematopoietic, or skeletal myogenic progenitors in a context-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9BRJ9()