Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) [Janelia Fluor® 525]

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: A103 + T311 + HMB45
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) [Janelia Fluor® 525] Summary

• Immunogen: Recombinant Melanoma Marker (MART-1 + Tyrosinase + gp100) protein (A103); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
• Localization: Cytoplasmic
• Marker: Melanoma Marker
• Specificity: This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
• Isotype: IgG1 Kappa/IgG2a Kappa/IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MLANA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Array

• MART1
• MART-1
• melan-A
• MLANA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MLANA