Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
M2-7C10 + M2-9E3
Clonality
Monoclonal
Host
Mouse

Melan-A/MART-1 Antibody (M2-7C10 M2-9E3) [Alexa Fluor® 488] Summary

Immunogen
Recombinant hMART-1 protein (M2-7C10; M2-9E3)
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
MLANA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Alexa Fluor 488
Format
Alexa Fluor 488 (A=488, E=515-545)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Alternate Names for Melan-A/MART-1 Antibody (M2-7C10 M2-9E3) [Alexa Fluor® 488]

  • Antigen LB39-AA
  • Antigen SK29-AA
  • Mart 1 Melan A
  • MART-1
  • MART1MART-1
  • MelanA
  • melan-A
  • Melan-A/MART-1
  • melanoma antigen recognized by T-cells 1
  • MLANA
  • Protein Melan-A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Melan-A/MART-1 NBP2-34680AF488

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol MLANA
Entrez

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