MEKK3 Antibody (576240) [Janelia Fluor® 646]

• Reactivity: Hu
• Applications: WB, KO
• Clone: 576240
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 646

MEKK3 Antibody (576240) [Janelia Fluor® 646] Summary

• Immunogen: E. coli-derived recombinant human MEKK3
  Ser43-Tyr275
  Accession # Q99759
• Specificity: Detects human MEKK3 in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAP3K3
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MEKK3 Antibody (576240) [Janelia Fluor® 646]

• EC 2.7.11.25
• MAP/ERK kinase kinase 3
• MAP3K3
• MAPK/ERK kinase kinase 3
• MAPKKK3
• MAPKKK3MEKK 3
• MEK kinase 3
• MEKK3
• mitogen-activated protein kinase kinase kinase 3

Background

MEKK3 is a Ser/Thr protein kinase belonging to the MAP/ERK kinase kinase family (1). Closely related to MEKK2, MEKK3 is expressed in a multitude of tissues. MEKK3 directly regulates SAPKs and ERKs pathway via SEK and MEK1, respectively but not the p38 pathway (2) and is also involved in the NF-kB pathway (3). MEKK3 as well as MEKK2 bind, phosphorylate and activate MEK5 through their PB1 domain (4). hKSR-2 (human kinase suppressor of ras-2) has been shown to regulate the activity of MEKK3 (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP3K3