Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were ...read more
Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer218,222 of human MEK 1/2. Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221.
p Ser218, p Ser222
Specific for the ~45k MEK 1/2 protein phosphorylated at Ser218/222 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Human (100%), Rat (100%), Primate (100%), Xenopus (100%), Bovine (100%), Zebrafish (100%), Canine (100%), Chicken (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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Alternate Names for MEK1 [p Ser218, p Ser222] Antibody
dual specificity mitogen-activated protein kinase kinase 1
ERK activator kinase 1
MAP kinase kinase 1
MAPK/ERK kinase 1
mitogen-activated protein kinase kinase 1
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Microtubule-associated protein kinase kinase 1 + 2 are members of a tyrosine/threonine protein kinase family that activate the ERK 1 + 2/MAPK enzymes by phosphorylating both residues within the threonine
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for MEK1 Antibody (NB500-139). (Showing 1 - 1 of 1 FAQ).
Would you please help confirm if NB500-139 can recognize both MEK1 and MEK2 protein phosphorylated at Ser218/222? Although the product description shows that NB500-139 shall react with MEK 1/2, the product name only mentions MEK1.
NB500-139 will recognize both MEK1 and MEK2, however do keep in mind that they are 45 and 47 kDa respectively and may be hard to distinguish on a western blot.
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