MEK1 [p Ser218, p Ser222] Antibody

Images

 
Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, Hu, Rt, Bv, Ca, Ch, Pm, Xp, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

MEK1 [p Ser218, p Ser222] Antibody Summary

Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer218,222 of human MEK 1/2. Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221.
Modification
p Ser218, p Ser222
Specificity
Specific for the ~45k MEK 1/2 protein phosphorylated at Ser218/222 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Predicted Species
Human (100%), Rat (100%), Primate (100%), Xenopus (100%), Bovine (100%), Zebrafish (100%), Canine (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP2K1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
This antibody is useful for Western blot where a band at 45 kDa is seen.
Publications
Read Publication using NB500-139.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
0.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MEK1 [p Ser218, p Ser222] Antibody

  • dual specificity mitogen-activated protein kinase kinase 1
  • ERK activator kinase 1
  • MAP kinase kinase 1
  • MAP2K1
  • MAPK/ERK kinase 1
  • MAPKK1
  • MEK1
  • MEK1MAPKK 1
  • mitogen-activated protein kinase kinase 1
  • MKK1
  • MKK1MEK 1
  • PRKMK1EC 2.7.12.2
  • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

Microtubule-associated protein kinase kinase 1 + 2 are members of a tyrosine/threonine protein kinase family that activate the ERK 1 + 2/MAPK enzymes by phosphorylating both residues within the threonine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MEK1 Antibody (NB500-139)(1)

We have publications tested in 1 confirmed species: Human.


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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MEK1 Antibody (NB500-139). (Showing 1 - 1 of 1 FAQ).

  1. Would you please help confirm if NB500-139 can recognize both MEK1 and MEK2 protein phosphorylated at Ser218/222? Although the product description shows that NB500-139 shall react with MEK 1/2, the product name only mentions MEK1.
    • NB500-139 will recognize both MEK1 and MEK2, however do keep in mind that they are 45 and 47 kDa respectively and may be hard to distinguish on a western blot.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP2K1
Entrez
Uniprot