MEK1 [p Ser218, p Ser222] Antibody - Azide Free

Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Pm, Xp, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

MEK1 [p Ser218, p Ser222] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit MEK1 [p Ser218, p Ser222] Antibody - Azide Free (NB500-139) is a polyclonal antibody validated for use in WB. Anti-MEK1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 conjugated to KLH. Accession # Q02750
• Modification: p Ser218, p Ser222
• Specificity: Specific for endogenous levels of the ~45 kDa MEK1 protein phosphorylated at Ser218/222. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
• Predicted Species: Rat (100%), Chicken (100%), Canine (100%), Xenopus (100%), Primate (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP2K1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: This antibody is useful for Western blot where a band at 45 kDa is seen.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for MEK1 [p Ser218, p Ser222] Antibody - Azide Free

• dual specificity mitogen-activated protein kinase kinase 1
• ERK activator kinase 1
• MAP kinase kinase 1
• MAP2K1
• MAPK/ERK kinase 1
• MAPKK1
• MEK1
• MEK1MAPKK 1
• mitogen-activated protein kinase kinase 1
• MKK1
• MKK1MEK 1
• PRKMK1EC 2.7.12.2
• protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

Microtubule-associated protein kinase kinase 1 + 2 are members of a tyrosine/threonine protein kinase family that activate the ERK 1 + 2/MAPK enzymes by phosphorylating both residues within the threonine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MEK1 Antibody (NB500-139)(1)

Publication using NB500-139

• Chen JQ, Lee JH, Herrmann MA et al. Capillary isoelectric-focusing immunoassays to study dynamic oncoprotein phosphorylation and drug response to targeted therapies in non-small cell lung cancer. Mol Cancer Ther. 2013-11-01 [PMID: 23979919] (Human)

FAQs for MEK1 Antibody (NB500-139). (Showing 1 - 1 of 1 FAQ).

1. Would you please help confirm if NB500-139 can recognize both MEK1 and MEK2 protein phosphorylated at Ser218/222? Although the product description shows that NB500-139 shall react with MEK 1/2, the product name only mentions MEK1.
   • NB500-139 will recognize both MEK1 and MEK2, however do keep in mind that they are 45 and 47 kDa respectively and may be hard to distinguish on a western blot.

Bioinformatics

• Gene Symbol: MAP2K1
• Entrez:
  • 26395(Mouse)
  • 170851(Rat)
• Uniprot:
  • Q02750()