MEK1 [p Thr292] Antibody (SR1773) - BSA Free

Western Blot: MEK1 [p Thr 292] Antibody (SR1773) [NBP3-21559] - Western blot analysis using NBP3-21559 in (1) HeLa cell lysate; (2) HeLa cell treated with Nocodazole.

• Reactivity: Hu
• Applications: WB
• Clone: SR1773
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MEK1 [p Thr292] Antibody (SR1773) - BSA Free Summary

• Description: Novus Biologicals Rabbit MEK1 [p Thr292] Antibody (SR1773) - BSA Free (NBP3-21559) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-MEK1 [Thr292] (Uniprot #: Q02750 )
• Modification: p Thr292
• Specificity: Detects endogenous levels of phospho-MEK1 [Thr292]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAP2K1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MEK1 [p Thr292] Antibody (SR1773) - BSA Free

• dual specificity mitogen-activated protein kinase kinase 1
• ERK activator kinase 1
• MAP kinase kinase 1
• MAP2K1
• MAPK/ERK kinase 1
• MAPKK1
• MEK1
• MEK1MAPKK 1
• mitogen-activated protein kinase kinase 1
• MKK1
• MKK1MEK 1
• PRKMK1EC 2.7.12.2
• protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K1