MEGF9 Antibody

Western blot shows lysates of IMR-32 human neuroblastoma cell line and human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MEGF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MEGF9 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MEGF9 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human MEGF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7768) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

MEGF9 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MEGF9
  Ala36-Asn514
  Accession # Q9H1U4
• Specificity: Detects human MEGF9 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: MEGF9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MEGF9 Antibody

• EGFL5
• EGFL5KIAA0818
• EGF-like protein 5
• EGF-like-domain, multiple 5
• Epidermal growth factor-like protein 5
• MEGF9
• Multiple EGF-like domains protein 9
• multiple EGF-like-domains 9
• multiple epidermal growth factor-like domains protein 9

Background

MEGF9 (Multiple EGF‑like domains protein 9; also EGF‑like protein 5) is a 63 kDa (predicted) novel transmembrane glycoprotein that shares some homology to beta ‑chains of laminin. It is expressed by hepatocytes, cerebellar Purkinje cells, Schwann cells, keratinocytes and intestinal epithelium. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration. Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular region (aa 31‑514) plus a 68 aa C‑terminal cytoplasmic domain. The extracellular region possesses a lengthy Pro‑rich region (aa 55‑200), followed by five EGF‑like domains (aa 204‑451). MEGF9 may run at approximately 160 kDa in SDS‑PAGE, suggesting either heavy glycosylation or dimerization. Over aa 36‑514, human MEGF9 shares 76% aa sequence identity with mouse MEGF9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MEGF9 Antibody (AF7768)(1)

Publication using AF7768

• Heller B, Ghidinelli M, Voelkl J, Einheber S, Smith R, Grund E, Morahan G, Chandler D, Kalaydjieva L, Giancotti F, King R, Fejes-Toth A, Fejes-Toth G, Feltri M, Lang F, Salzer J Functionally distinct PI 3-kinase pathways regulate myelination in the peripheral nervous system. J Cell Biol, 2014-03-31;204(7):1219-36. 2014-03-31 [PMID: 24687281] (Bioassay, Mouse, Rat)

Bioinformatics

• Gene Symbol: MEGF9
• Uniprot:
  • Q9H1U4(Human)