MEGF9 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

MEGF9 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MEGF9
  Ala36-Asn514
  Accession # Q9H1U4
• Specificity: Detects human MEGF9 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MEGF9 Antibody [Alexa Fluor® 488]

• EGFL5
• EGFL5KIAA0818
• EGF-like protein 5
• EGF-like-domain, multiple 5
• Epidermal growth factor-like protein 5
• MEGF9
• Multiple EGF-like domains protein 9
• multiple EGF-like-domains 9
• multiple epidermal growth factor-like domains protein 9

Background

MEGF9 (Multiple EGF‑like domains protein 9; also EGF‑like protein 5) is a 63 kDa (predicted) novel transmembrane glycoprotein that shares some homology to beta ‑chains of laminin. It is expressed by hepatocytes, cerebellar Purkinje cells, Schwann cells, keratinocytes and intestinal epithelium. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration. Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular region (aa 31‑514) plus a 68 aa C‑terminal cytoplasmic domain. The extracellular region possesses a lengthy Pro‑rich region (aa 55‑200), followed by five EGF‑like domains (aa 204‑451). MEGF9 may run at approximately 160 kDa in SDS‑PAGE, suggesting either heavy glycosylation or dimerization. Over aa 36‑514, human MEGF9 shares 76% aa sequence identity with mouse MEGF9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.