MED4 Antibody - BSA Free

Western Blot: MED4 Antibody [NBP1-33671] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: MED4 Antibody [NBP1-33671] - Paraformaldehyde-fixed A549, using antibody at 1:500 dilution.

• Reactivity: Hu, RM
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MED4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MED4 Antibody - BSA Free (NBP1-33671) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MED4. The exact sequence is proprietary.
• Localization: Nucleus |
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MED4 Antibody - BSA Free

• Activator-recruited cofactor 36 kDa component
• ARC36
• DRIP36
• DRIP36mediator of RNA polymerase II transcription, subunit 4 homolog (S. cerevisiae)
• FLJ10956
• HSPC126
• HSPC126vitamin D receptor interacting protein
• MED4
• mediator complex subunit 4TRAP/SMCC/PC2 subunit p36 subunit
• subunit 4 homolog
• TRAP36
• VDRIP

Background

MED4 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MED4