MED27 Antibody

Images

 
Western Blot: MED27 Antibody [H00009442-B01P] - Analysis of MED27 expression in transfected 293T cell line by MED27 polyclonal antibody. Lane 1: CRSP8 transfected lysate(34.21 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MED27 Antibody Summary

Immunogen
MED27 (NP_004260.2, 1 a.a. ~ 311 a.a) full-length human protein.MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGKLDIWSKSNYQVFQKVTDHATTALLHYQL
Specificity
MED27 - mediator complex subunit 27,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
MED27
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED27 Antibody

  • cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa
  • CRSP34CRSP complex subunit 8
  • CRSP8CRAP34
  • mediator complex subunit 27Transcriptional coactivator CRSP34
  • mediator of RNA polymerase II transcription subunit 27
  • MGC11274
  • P37 TRAP/SMCC/PC2 subunit
  • subunit 8 (34kD)
  • TRAP37

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MED27
Entrez
Uniprot