MED27 Antibody - Azide and BSA Free

Western Blot: MED27 Antibody [H00009442-B01P] - Analysis of MED27 expression in transfected 293T cell line by MED27 polyclonal antibody. Lane 1: CRSP8 transfected lysate(34.21 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MED27 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse MED27 Antibody - Azide and BSA Free (H00009442-B01P) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: MED27 (NP_004260.2, 1 a.a. - 311 a.a.) full-length human protein. MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGKLDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ
• Specificity: MED27 - mediator complex subunit 27,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: MED27
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED27 Antibody - Azide and BSA Free

• cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa
• CRSP34CRSP complex subunit 8
• CRSP8CRAP34
• mediator complex subunit 27Transcriptional coactivator CRSP34
• mediator of RNA polymerase II transcription subunit 27
• MGC11274
• P37 TRAP/SMCC/PC2 subunit
• subunit 8 (34kD)
• TRAP37

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED27
• Entrez:
  • 9442(Human)
• Uniprot:
  • NP_004260.2(Human)