MED29 Antibody - Azide and BSA Free

Western Blot: MED29 Antibody [H00055588-B01P] - Analysis of MED29 expression in transfected 293T cell line by MED29 polyclonal antibody. Lane 1: MED29 transfected lysate(24.31 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MED29 Antibody [H00055588-B01P] - Analysis of purified antibody to MED29 on HeLa cell. (antibody concentration 10 ug/ml)

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MED29 Antibody - Azide and BSA Free Summary

• Immunogen: MED29 (NP_060062, 1 a.a. - 221 a.a.) full-length human protein. MLKSNGERRSRNALPAVYARKMAASQQQASAASSAAGVSGPSSAGGPGPQQQPQPPAQLVGPAQSGLLQQQQQDFDPVQRYKMLIPQLKESLQTLMKVAAQNLIQNTNIDNGQKSSDGPIQRFDKCLEEFYALCDQLELCLRLAHECLSQSCDSAKHSPTLVPTATKPDAVQPDSLPYPQYLAVIKAQISCAKDIHTALLDCANKVTGKTPAPPAGPGGTL
• Specificity: IXL - intersex-like (Drosophila),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: MED29
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It has also been used for immunofluorescence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED29 Antibody - Azide and BSA Free

• intersex-like (Drosophila)
• Intersex-like protein
• mediator complex subunit 29IXLDKFZp434H247
• mediator of RNA polymerase II transcription subunit 29

Background

MED29 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 (PubMed 14576168)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED29
• Entrez:
  • 55588(Human)
• Uniprot:
  • NP_060062(Human)