MED25 Antibody - BSA Free

Western Blot: MED25 Antibody [NBP2-55868] - Analysis in human cell line RT-4 and human cell line U-251 MG.

Immunocytochemistry/Immunofluorescence: MED25 Antibody [NBP2-55868] - Staining of human cell line A549 shows localization to nucleoplasm. Antibody staining is shown in green.

ChIP-Exo-Seq composite graph for Anti-MED25 (NBP2-55868) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MED25 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MED25 Antibody - BSA Free (NBP2-55868) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-MED25 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPG
• Predicted Species: Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED25
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq 1-10ug per reaction
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MED25 Recombinant Protein Antigen (NBP2-55868PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MED25 Antibody - BSA Free

• ACID1
• Activator Interaction Domain-Containing Protein 1
• Activator-Recruited Cofactor 92 KDa Component
• ARC/Mediator Transcriptional Coactivator Subunit
• ARC92
• BVSYS
• CMT2B2
• Mediator Complex Subunit 25
• Mediator Of RNA Polymerase II Transcription Subunit 25
• Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog (S. Cerevisiae)
• Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog
• P78
• TCBAP0758

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MED25 Antibody (NBP2-55868)(2)

Publications using NBP2-55868

• Tessa Van Royen, Koen Sedeyn, George D. Moschonas, Wendy Toussaint, Marnik Vuylsteke, Delphi Van Haver, Francis Impens, Sven Eyckerman, Irma Lemmens, Jan Tavernier, Bert Schepens, Xavier Saelens, Rebecca Ellis Dutch An Unexpected Encounter: Respiratory Syncytial Virus Nonstructural Protein 1 Interacts with Mediator Subunit MED25 Journal of Virology 2022-09-14 [PMID: 36102648]
• Garlick J Innovations in Targeting Dynamic Proteins With Small Molecule Modulators Thesis 2021-01-01 (WB)

Bioinformatics

• Gene Symbol: MED25